Academic Journal
mRNA level of antioxidant genes and activity of NADPH-generating enzymes in rotenone-induced parkinsonism in rats ; Уровень мРНК генов антиоксидантной системы и активность НАДФН-генерирующих ферментов при ротенон-индуцированном паркинсонизме у крыс
| العنوان: | mRNA level of antioxidant genes and activity of NADPH-generating enzymes in rotenone-induced parkinsonism in rats ; Уровень мРНК генов антиоксидантной системы и активность НАДФН-генерирующих ферментов при ротенон-индуцированном паркинсонизме у крыс |
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| المؤلفون: | E. D. Kryl’skii, G. A. Razuvaev, T. N. Popova, L. E. Nikhaev, A. I. Akinina, Е. Д. Крыльский, Г. А. Разуваев, Т. Н. Попова, Л. Е. Нихаев, А. И. Акинина |
| المصدر: | Bulletin of Siberian Medicine; Том 22, № 2 (2023); 78-87 ; Бюллетень сибирской медицины; Том 22, № 2 (2023); 78-87 ; 1819-3684 ; 1682-0363 ; 10.20538/1682-0363-2023-22-2 |
| بيانات النشر: | Siberian State Medical University, the Ministry of Healthcare of the Russian Federation |
| سنة النشر: | 2023 |
| المجموعة: | Bulletin of Siberian Medicine / Бюллетень сибирской медицины |
| مصطلحات موضوعية: | НАДФ-изоцитратдегидрогеназа, oxidative stress, antioxidant system, glucose-6-phosphate dehydrogenase, NADPdependent isocitrate dehydrogenase, окислительный стресс, антиоксидантная система, глюкозо-6-фосфатдегидрогеназа |
| الوصف: | Aim. To analyze the mRNA level of genes encoding antioxidant enzymes and the transcription factors Nrf2 and Foxo1 regulating their expression and the activity of glucose-6-phosphate dehydrogenase (G6PDH) and NADPdependent isocitrate dehydrogenase (NADP-IDH) and assess the correlation between these parameters, oxidative status, and motor coordination parameters in rats with rotenone-induced parkinsonism.Materials and methods. The study was performed on male Wistar rats aged 4–6 months and weighing 200–250 g. Parkinsonism was modeled by subcutaneous administration of rotenone for 10 days at a dose of 2.5 mg / kg. To confirm the development of the pathology, motor coordination tests and histological staining of the cerebral cortex and striatum with hematoxylin and eosin were used. The oxidative status was analyzed based on the levels of conjugated dienes, carbonyl amino acid residues in proteins, and α-tocopherol. The enzyme activity was studied spectrophotometrically by the formation of NADPH. Real-time PCR was used to analyze the level of gene mRNA.Results. During the study, an increase in serum and brain concentrations of conjugated dienes, carbonyl amino acid residues, and α-tocopherol was observed in the experimental group of rats compared to the controls. It could be associated with the redistribution of this compound between tissues during pathology development. The animals with experimental parkinsonism, in addition, were characterized by a decrease in the mRNA level of the Sod1, Gpx1, Gsr, Gsta2, Nfe2l2, and Foxo1 genes, as well as the activity of G6PDH and NADP-IDH. In the rats with experimental parkinsonism, a negative correlation of NADPH-IDH activity in the brain with serum α-tocopherol level and a positive correlation with Gpx1 and Foxo1 mRNA levels in the striatum were found. The level of oxidatively modified proteins in the brain of the animals with PD was negatively correlated with the concentration of Gsta2 mRNA in the striatum, while the specific activity of G6PDH in the serum was characterized ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Russian English |
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| DOI: | 10.20538/1682-0363-2023-2-78-87 |
| الاتاحة: | https://bulletin.ssmu.ru/jour/article/view/5224 https://doi.org/10.20538/1682-0363-2023-2-78-87 |
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| رقم الانضمام: | edsbas.75163A92 |
| قاعدة البيانات: | BASE |
| DOI: | 10.20538/1682-0363-2023-2-78-87 |
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