التفاصيل البيبلوغرافية
| العنوان: |
Functional and structural characterization of a novel isoamylase from ostreococcus tauri and role of the n-terminal domain |
| المؤلفون: |
Hedin, Nicolas, Barchiesi, Julieta, Gomez Casati, Diego Fabian, Busi, María Victoria |
| بيانات النشر: |
Bentham Science Publishers |
| المجموعة: |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: |
AMINO ACID RESIDUES, BIOCHEMICAL CHARACTERIZATION, CARBOHYDRATE BINDING DOMAIN (CBM), CARBOHYDRATES, DEBRANCHING STARCH ENZYMES, POLYSACCHARIDES, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/1 |
| الوصف: |
Background: The debranching starch enzymes, isoamylase 1 and 2 are well-conserved enzymes present in almost all the photosynthetic organisms. These enzymes are involved in the crystallization process of starch and are key components which remove misplaced α-1,6 ramifications on the final molecule. Aim: In this work, we performed a functional and structural study of a novel isoamylase from Ostreococcus tauri. Methods: We identified conserved amino acid residues possibly involved in catalysis. We also identified a region at the N-terminal end that resembles a Carbohydrate Binding Domain (CBM), which is more related to the family CBM48, but has no spatial conservation of the residues involved in carbohydrate binding. Results: The cloning, expression and biochemical characterization of this N-terminal region confirmed that it binds to polysaccharides, showing greater capacity for binding to amylopectin rather than total starch or amylose. Conclusion: This module could be a variant of the CBM48 family or it could be classified within a new CBM family. ; Fil: Hedin, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina ; Fil: Barchiesi, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina ; Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/url/https://openbiotechnologyjournal.com/VOLUME/14/PAGE/1/; http://hdl.handle.net/11336/173498; CONICET Digital; CONICET |
| الاتاحة: |
http://hdl.handle.net/11336/173498 |
| Rights: |
info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/2.5/ar/ |
| رقم الانضمام: |
edsbas.751164CC |
| قاعدة البيانات: |
BASE |