Academic Journal
The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators
| العنوان: | The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators |
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| المؤلفون: | Llorian, Miriam, Gooding, Clare, Bellora, Nicolás, Hallegger, Martina, Buckroyd, Adrian, Wang, Xiao, Rajgor, Dipen, Kayikci, Melis, Feltham, Jack, Ule, Jernej, Eyras, Eduardo, Smith, Christopher |
| بيانات النشر: | Oxford University Press |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | Alternative splicing, Cellular differentiation, Bioinformatics, Gene expression, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/1 |
| الوصف: | Alternative splicing (AS) is a key component of gene expression programs that drive cellular differentiation. Smooth muscle cells (SMCs) are important in the function of a number of physiological systems; however, investigation of SMC AS has been restricted to a handful of events. We profiled transcriptome changes in mouse de-differentiating SMCs and observed changes in hundreds of AS events. Exons included in differentiated cells were characterized by particularly weak splice sites and by upstream binding sites for Polypyrimidine Tract Binding protein (PTBP1). Consistent with this, knockdown experiments showed that that PTBP1 represses many smooth muscle specific exons. We also observed coordinated splicing changes predicted to downregulate the expression of core components of U1 and U2 snRNPs, splicing regulators and other post-transcriptional factors in differentiated cells. The levels of cognate proteins were lower or similar in differentiated compared to undifferentiated cells. However, levels of snRNAs did not follow the expression of splicing proteins, and in the case of U1 snRNP we saw reciprocal changes in the levels of U1 snRNA and U1 snRNP proteins. Our results suggest that the AS program in differentiated SMCs is orchestrated by the combined influence of auxiliary RNA binding proteins, such as PTBP1, along with altered activity and stoichiometry of the core splicing machinery. ; Fil: Llorian, Miriam. University of Cambridge; Reino Unido ; Fil: Gooding, Clare. University of Cambridge; Reino Unido ; Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. University College London; Reino Unido. Institució Catalana de Recerca i Estudis Avancats; España ; Fil: Hallegger, Martina. University College London; Estados ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0305-1048 1362-4962 |
| Relation: | info:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/early/2016/06/17/nar.gkw560.full.pdf+html; info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/44/18/8933/2468335; http://hdl.handle.net/11336/74313; Llorian, Miriam; Gooding, Clare; Bellora, Nicolás; Hallegger, Martina; Buckroyd, Adrian; et al.; The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators; Oxford University Press; Nucleic Acids Research; 44; 18; 17-10-2016; 8933-8950; CONICET Digital; CONICET |
| الاتاحة: | http://hdl.handle.net/11336/74313 |
| Rights: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/2.5/ar/ |
| رقم الانضمام: | edsbas.74FA67C9 |
| قاعدة البيانات: | BASE |
| تدمد: | 03051048 13624962 |
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