Expression of fibroblast activation protein-α in human deep vein thrombosis
| العنوان: | Expression of fibroblast activation protein-α in human deep vein thrombosis |
|---|---|
| المؤلفون: | Oguri, Nobuyuki, 魏, 峻洸, 34464, ギ, トシヒロ, 30794656, Gi, Toshihiro, 中村, 恵理子, 34710, ナカムラ, エリコ, 20981088, Nakamura, Eriko, 古小路, 英二, 24909, フルコウジ, エイジ, 00423723, Furukoji, Eiji, 後藤, 洋規, 12514, ゴトウ, ヒロキ, Goto, Hiroki, Maekawa, Kazunari, 34339, 前川, 和也, マエカワ, カズナリ, Tsuji, Atsushi B., Nishii, Ryuichi, 阿萬, 紫, 34712, アマン, ムラサキ, 50444790, Aman, Murasaki, Moriguchi, Sayaka, 盛口, 清香, 90468041, モリグチ, サヤカ, 榮, 建文, 35185, サカエ, タテフミ, 20347074, Sakae, Tatefumi, 東, 美菜子, 34675, アズマ, ミナコ, 00643389, Azuma, Minako, 山下, 篤, ヤマシタ, アツシ, 90372797, Yamashita, Atsushi |
| بيانات النشر: | Elsevier BV |
| المجموعة: | University of Miyazaki: Dspace |
| مصطلحات موضوعية: | α2-antiplasmin, Deep vein thrombosis, Fibroblast, Fibroblast activation protein-α, Organization, Podoplanin |
| الوصف: | Background Fibroblast activation protein-α (FAP), a type-II transmembrane serine protease, is associated with wound healing, cancer-associated fibroblasts, and chronic fibrosing diseases. However, its expression in deep vein thrombosis (DVT) remains unclear. Therefore, this study investigated FAP expression and localization in DVT. Methods We performed pathological analyses of the aspirated thrombi of patients with DVT (n = 14), classifying thrombotic areas in terms of fresh, cellular lysis, and organizing reaction components. The organizing reaction included endothelialization and fibroblastic reaction. We immunohistochemically examined FAP-expressed areas and cells, and finally analyzed FAP expression in cultured dermal fibroblasts. Results All the aspirated thrombi showed a heterogeneous mixture of at least two of the three thrombotic areas. Specifically, 83 % of aspirated thrombi showed fresh and organizing reaction components. Immunohistochemical expression of FAP was restricted to the organizing area. Double immunofluorescence staining showed that FAP in the thrombi was mainly expressed in vimentin-positive or α-smooth muscle actin-positive fibroblasts. Some CD163-positive macrophages expressed FAP. FAP mRNA and protein levels were higher in fibroblasts with low-proliferative activity cultured under 0.1 % fetal bovine serum (FBS) than that under 10 % FBS. Fibroblasts cultured in 10 % FBS showed a significant decrease in FAP mRNA levels following supplementation with hemin, but not with thrombin. Conclusions The heterogeneous composition of venous thrombi suggests a multistep thrombus formation process in human DVT. Further, fibroblasts or myofibroblasts may express FAP during the organizing process. FAP expression may be higher in fibroblasts with low proliferative activity. ; journal article |
| نوع الوثيقة: | other/unknown material |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Thrombosis Research; 241; 109075; http://hdl.handle.net/10458/0002000874 |
| الاتاحة: | https://www.sciencedirect.com/science/article/pii/S004938482400207X/pdfft?md5=73ac63c826d44103e3e2c69276ce5574&pid=1-s2.0-S004938482400207X-main.pdf http://hdl.handle.net/10458/0002000874 https://miyazaki-u.repo.nii.ac.jp/records/2000874 |
| Rights: | © 2024 The Authors. Published by Elsevier Ltd. ; open access |
| رقم الانضمام: | edsbas.749DBDCB |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |