Academic Journal
Ferulic acid-loaded polymeric nanoparticles prepared from nano-emulsion templates facilitate internalisation across the blood-brain barrier in model membranes
| العنوان: | Ferulic acid-loaded polymeric nanoparticles prepared from nano-emulsion templates facilitate internalisation across the blood-brain barrier in model membranes |
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| المؤلفون: | Garcia, Luna, Palma-Florez, Sujey, Espinosa, Victor, Soleimani Rokni, Fatemeh, Lagunas, Anna, Mir, Mònica, García-Celma, M. J., Samitier, Josep, Rodríguez-Abreu, Carlos, Grijalvo, Santiago |
| المساهمون: | orcid |
| بيانات النشر: | Royal Society of Chemistry (UK) |
| سنة النشر: | 2023 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Ferulic acid, Polymeric nanoparticles |
| الوصف: | A hydroxycinnamic acid derivative, namely ferulic acid (FA) has been successfully encapsulated in polymeric nanoparticles (NPs) based on poly(lactic-co-glycolic acid) (PLGA). FA-loaded polymeric NPs were prepared from O/W nano-emulsion templates using the phase inversion composition (PIC) low-energy emulsification method. The obtained PLGA NPs exhibited high colloidal stability, good drug-loading capacity, and particle hydrodynamic diameters in the range of 74 to 117 nm, depending on the FA concentration used. In vitro drug release studies confirmed a diffusion-controlled mechanism through which the amount of released FA reached a plateau at 60% after 6 hours-incubation. Five kinetic models were used to fit the FA release data as a function of time. The Weibull distribution and Korsmeyer-Peppas equation models provided the best fit to our experimental data and suggested quasi-Fickian diffusion behaviour. Moderate dose-response antioxidant and radical scavenging activities of FA-loaded PLGA NPs were demonstrated using the DPPH˙ assay achieving inhibition activities close to 60 and 40%, respectively. Cell culture studies confirmed that FA-loaded NPs were not toxic according to the MTT colorimetric assay, were able to internalise efficiently SH-SY5Y neuronal cells and supressed the intracellular ROS-level induced by H2O2 leading to 52% and 24.7% of cellular viability at 0.082 and 0.041 mg mL-1, respectively. The permeability of the NPs through the blood brain barrier was tested with an in vitro organ-on-a-chip model to evaluate the ability of the FA-loaded PLGA and non-loaded PLGA NPs to penetrate to the brain. NPs were able to penetrate the barrier, but permeability decreased when FA was loaded. These results are promising for the use of loaded PLGA NPs for the management of neurological diseases. ; The authors acknowledge the Agencia Estatal de Investigación for funding (Projects CTQ2017-84998-P and PID2021122187NB-C31) and the Instituto de Salud Carlos III (ISCIII) (CB06/01/1058 and CB06/01/0055). CIBER BBN is ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 20403364 |
| Relation: | Nanoscale; Publisher's version; https://doi.org/10.1039/D2NR07256D; Sí; Nanoscale (2023); http://hdl.handle.net/10261/307833; 2-s2.0-85153284674; https://api.elsevier.com/content/abstract/scopus_id/85153284674 |
| DOI: | 10.1039/d2nr07256d |
| الاتاحة: | http://hdl.handle.net/10261/307833 https://doi.org/10.1039/d2nr07256d https://api.elsevier.com/content/abstract/scopus_id/85153284674 |
| Rights: | open |
| رقم الانضمام: | edsbas.740CA508 |
| قاعدة البيانات: | BASE |
| تدمد: | 20403364 |
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| DOI: | 10.1039/d2nr07256d |