Academic Journal
Usefulness of [ 18 F]-DA and [ 18 F]-DOPA for PET imaging in a mouse model of pheochromocytoma
| العنوان: | Usefulness of [ 18 F]-DA and [ 18 F]-DOPA for PET imaging in a mouse model of pheochromocytoma |
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| المؤلفون: | Martiniova, Lucia, Cleary, Susannah, Lai, Edwin W., Kiesewetter, Dale O., Seidel, Jurgen, Dawson, Linda F., Phillips, Jacqueline K., Thomasson, David, Chen, Xiaoyuan, Eisenhofer, Graeme, Powers, James F., Kvetnansky, Richard, Pacak, Karel |
| المصدر: | Martiniova , L , Cleary , S , Lai , E W , Kiesewetter , D O , Seidel , J , Dawson , L F , Phillips , J K , Thomasson , D , Chen , X , Eisenhofer , G , Powers , J F , Kvetnansky , R & Pacak , K 2012 , ' Usefulness of [ 18 F]-DA and [ 18 F]-DOPA for PET imaging in a mouse model of pheochromocytoma ' , Nuclear Medicine and Biology , vol. 39 , no. 2 , pp. 215-226 . https://doi.org/10.1016/j.nucmedbio.2011.07.007 |
| سنة النشر: | 2012 |
| الوصف: | Purpose: To evaluate the usefulness of [ 18 F]-6-fluorodopamine ([ 18 F]-DA) and [ 18 F]-L-6-fluoro-3,4-dihydroxyphenylalanine ([ 18 F]-DOPA) positron emission tomography (PET) in the detection of subcutaneous (s.c.) and metastatic pheochromocytoma in mice; to assess the expression of the norepinephrine transporter (NET) and vesicular monoamine transporters 1 and 2 (VMAT1 and VMAT2), all important for [ 18 F]-DA and [ 18 F]-DOPA uptake. Furthermore, to compare tumor detection by micro-computed tomography (microCT) to magnetic resonance imaging (MRI) in individual mouse. Methods: SUV max values were calculated from [ 18 F]-DA and [ 18 F]-DOPA PET, tumor-to-liver ratios (TLR) were obtained and expression of NET, VMAT1 and VMAT2 was evaluated. Results: [ 18 F]-DA detected less metastatic lesions compared to [ 18 F]-DOPA. TLR values for liver metastases were 2.26-2.71 for [ 18 F]-DOPA and 1.83-2.83 for [ 18 F]-DA. A limited uptake of [ 18 F]-DA was found in s.c. tumors (TLR=0.22-0.27) compared to [ 18 F]-DOPA (TLR=1.56-2.24). Overall, NET and VMAT2 were expressed in all organ and s.c. tumors. However, s.c. tumors lacked expression of VMAT1. We confirmed [ 18 F]-DA's high affinity for the NET for its uptake and VMAT1 and VMAT2 for its storage and retention in pheochromocytoma cell vesicles. In contrast, [ 18 F]-DOPA was found to utilize only VMAT2. Conclusion: MRI was superior in the detection of all organ tumors compared to microCT and PET. [ 18 F]-DOPA had overall better sensitivity than [ 18 F]-DA for the detection of metastases. Subcutaneous tumors were localized only with [ 18 F]-DOPA, a finding that may reflect differences in expression of VMAT1 and VMAT2, perhaps similar to some patients with pheochromocytoma where [ 18 F]-DOPA provides better visualization of lesions than [ 18 F]-DA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.nucmedbio.2011.07.007 |
| الاتاحة: | https://researchers.mq.edu.au/en/publications/f5c02b50-56d3-4a09-b82f-70b9bb7f640f https://doi.org/10.1016/j.nucmedbio.2011.07.007 http://www.scopus.com/inward/record.url?scp=84856340250&partnerID=8YFLogxK |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.73F29FBC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.nucmedbio.2011.07.007 |
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