Academic Journal
Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso.
| العنوان: | Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso. |
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| المؤلفون: | Barry, Aissata, Behet, Marije C, Nébié, Issa, Lanke, Kjerstin, Grignard, Lynn, Ouedraogo, Alphonse, Soulama, Issiaka, Drakeley, Chris, Sauerwein, Robert, Bolscher, Judith M, Dechering, Koen J, Bousema, Teun, Tiono, Alfred B, Gonçalves, Bronner P |
| بيانات النشر: | F1000Research |
| سنة النشر: | 2018 |
| المجموعة: | London School of Hygiene & Tropical Medicine: LSHTM Research Online |
| الوصف: | Background: Individuals living in malaria-endemic regions develop immunity against severe malaria, but it is unclear whether immunity against pre-erythrocytic stages that blocks initiation of blood-stage infection after parasite inoculation develops following continuous natural exposure. Methods: We cleared schoolchildren living in an area (health district of Saponé, Burkina Faso) with highly endemic seasonal malaria of possible sub-patent infections and examined them weekly for incident infections by nested PCR. Plasma samples collected at enrolment were used to quantify antibodies to the pre-eryhrocytic-stage antigens circumsporozoite protein (CSP) and Liver stage antigen 1 (LSA-1). In vitro sporozoite gliding inhibition and hepatocyte invasion inhibition by naturally acquired antibodies were assessed using Plasmodium falciparum NF54 sporozoites. Associations between antibody responses, functional pre-erythrocytic immunity phenotypes and time to infection detected by 18S quantitative PCR were studied. Results: A total of 51 children were monitored. Anti-CSP antibody titres showed a positive association with sporozoite gliding motility inhibition (P<0.0001, Spearman's ρ=0.76). In vitro hepatocyte invasion was inhibited by naturally acquired antibodies (median inhibition, 19.4% [IQR 15.2-40.9%]), and there were positive correlations between invasion inhibition and gliding inhibition (P=0.005, Spearman's ρ=0.67) and between invasion inhibition and CSP-specific antibodies (P=0.002, Spearman's ρ=0.76). Survival analysis indicated longer time to infection in individuals displaying higher-than-median sporozoite gliding inhibition activity (P=0.01), although this association became non-significant after adjustment for blood-stage immunity (P = 0.06). Conclusions: In summary, functional antibodies against the pre-erythrocytic stages of malaria infection are acquired in children who are repeatedly exposed to Plasmodium parasites. This immune response does not prevent them from becoming infected during a malaria ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://researchonline.lshtm.ac.uk/id/eprint/4653283/1/Barry-etal-2019-Functional-antibodies.pdf; Barry, Aissata; Behet, Marije C; Nébié, Issa; Lanke, Kjerstin; Grignard, Lynn ; Ouedraogo, Alphonse; Soulama, Issiaka; Drakeley, Chris ; Sauerwein, Robert; Bolscher, Judith M; +4 more. Dechering, Koen J; Bousema, Teun; Tiono, Alfred B; Gonçalves, Bronner P ; (2018) Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso. Wellcome open research, 3. 159-. DOI: https://doi.org/10.12688/wellcomeopenres.14932.2 |
| الاتاحة: | https://researchonline.lshtm.ac.uk/id/eprint/4653283/ https://researchonline.lshtm.ac.uk/id/eprint/4653283/1/Barry-etal-2019-Functional-antibodies.pdf |
| Rights: | cc_by |
| رقم الانضمام: | edsbas.73EC40E6 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |