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Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment

التفاصيل البيبلوغرافية
العنوان: Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment
المؤلفون: Rubio Pérez, Carlota, Planas Rigol, Ester, Trincado, Juan L., Bonfill Teixidor, Ester, Arias Piñeiro, Alexandra, Marchese, Domenica, Iurlaro, Raffaella, Martínez Ricarte, Francisco Ramón, Escudero Monreal, Laura, Cordero Asanza, Esteban Xavier, Cicuendez López Ocaña, Marta, Nuciforo, Paolo Giovanni, Sahuquillo Barris, Joan, Tabernero Caturla, Josep, Seoane Suarez, Joan, Serna, Garazi
المساهمون: Institut Català de la Salut, Rubio-Perez C, Planas-Rigol E, Bonfill-Teixidor E, Arias A, Serna G, Iurlaro R, Escudero L, Nuciforo P Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Trincado JL CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. Josep Carreras Leukemia Research Institute and Department of Biomedicine, School of Medicine, University of Barcelona, Barcelona, Spain. Marchese D CNAG-CRG, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. Martínez-Ricarte F, Cordero E, Cicuendez M, Sahuquillo J Vall d’Hebron Institut de Recerca (VHIR), Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Tabernero J Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. CIBERONC, Barcelona, Spain. Seoane J Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. CIBERONC, Barcelona, Spain. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus
المصدر: Scientia
بيانات النشر: Nature Research
سنة النشر: 2021
مصطلحات موضوعية: Cervell - Tumors, Càncer - Immunoteràpia, Metàstasi, DISEASES::Neoplasms::Neoplasms by Site::Nervous System Neoplasms::Central Nervous System Neoplasms::Brain Neoplasms, CHEMICALS AND DRUGS::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents::Antineoplastic Agents, Immunological, DISEASES::Neoplasms::Neoplastic Processes::Neoplasm Metastasis, ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias del sistema nervioso::neoplasias del sistema nervioso central::neoplasias cerebrales, COMPUESTOS QUÍMICOS Y DROGAS::acciones y usos químicos::acciones farmacológicas::usos terapéuticos::antineoplásicos::inmunoterapia antineoplásica, ENFERMEDADES::neoplasias::procesos neoplásicos::metástasis neoplásica
الوصف: Microambient del càncer; Immunologia tumoral ; Microambiente del cáncer; Inmunología tumoral ; Cancer microenvironment; Tumour immunology ; Brain metastases are the most common tumor of the brain with a dismal prognosis. A fraction of patients with brain metastasis benefit from treatment with immune checkpoint inhibitors (ICI) and the degree and phenotype of the immune cell infiltration has been used to predict response to ICI. However, the anatomical location of brain lesions limits access to tumor material to characterize the immune phenotype. Here, we characterize immune cells present in brain lesions and matched cerebrospinal fluid (CSF) using single-cell RNA sequencing combined with T cell receptor genotyping. Tumor immune infiltration and specifically CD8+ T cell infiltration can be discerned through the analysis of the CSF. Consistently, identical T cell receptor clonotypes are detected in brain lesions and CSF, confirming cell exchange between these compartments. The analysis of immune cells of the CSF can provide a non-invasive alternative to predict the response to ICI, as well as identify the T cell receptor clonotypes present in brain metastasis. ; The study was undertaken with the support of the Fundación Asociación Española contra el Cáncer (AECC), FERO (EDM), Ramón Areces Foundation, Cellex Foundation, BBVA (CAIMI), the ISCIII, FIS (PI16/01278), the Ministerio de Ciencia, Innovación y Universidades (SAF2017-89109-P; AEI/FEDER, UE), the Juan de la Cierva formación fellowship (C.R.-P. and L.E.), Sara Borrell fellowship (E.P-R.). We acknowledge support of the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa and the CERCA Program/Generalitat de Catalunya. We also acknowledge the support of the Spanish Ministry of Science and Innovation through the Instituto de Salud Carlos III, the Generalitat de Catalunya through Departament de Salut and Departament d’Empresa i Coneixement, and the Co-financing by the Spanish Ministry of Ministry of Science and ...
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
تدمد: 2041-1723
Relation: Nature Communications;12(1); https://www.nature.com/articles/s41467-021-21789-x; info:eu-repo/grantAgreement/ES/PE2013-2016/PI16%2F01278; info:eu-repo/grantAgreement/ES/PE2013-2016/SAF2017-89109-P; Rubio-Perez C, Planas-Rigol E, Trincado JL, Bonfill-Teixidor E, Arias A, Marchese D, et al. Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment. Nat Commun. 2021 Mar 8;12(1):1503.; https://hdl.handle.net/11351/6171; 000627442600001
DOI: 10.1038/s41467-021-21789-x
الاتاحة: https://hdl.handle.net/11351/6171
https://doi.org/10.1038/s41467-021-21789-x
Rights: Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess
رقم الانضمام: edsbas.73E328D6
قاعدة البيانات: BASE
الوصف
تدمد:20411723
DOI:10.1038/s41467-021-21789-x