Academic Journal
Serum response factor regulates smooth muscle contractility via myotonic dystrophy protein kinases and L-type calcium channels.
| العنوان: | Serum response factor regulates smooth muscle contractility via myotonic dystrophy protein kinases and L-type calcium channels. |
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| المؤلفون: | Moon Young Lee, Chanjae Park, Se Eun Ha, Paul J Park, Robyn M Berent, Brian G Jorgensen, Robert D Corrigan, Nathan Grainger, Peter J Blair, Orazio J Slivano, Joseph M Miano, Sean M Ward, Terence K Smith, Kenton M Sanders, Seungil Ro |
| المصدر: | PLoS ONE, Vol 12, Iss 2, p e0171262 (2017) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2017 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Serum response factor (SRF) transcriptionally regulates expression of contractile genes in smooth muscle cells (SMC). Lack or decrease of SRF is directly linked to a phenotypic change of SMC, leading to hypomotility of smooth muscle in the gastrointestinal (GI) tract. However, the molecular mechanism behind SRF-induced hypomotility in GI smooth muscle is largely unknown. We describe here how SRF plays a functional role in the regulation of the SMC contractility via myotonic dystrophy protein kinase (DMPK) and L-type calcium channel CACNA1C. GI SMC expressed Dmpk and Cacna1c genes into multiple alternative transcriptional isoforms. Deficiency of SRF in SMC of Srf knockout (KO) mice led to reduction of SRF-dependent DMPK, which down-regulated the expression of CACNA1C. Reduction of CACNA1C in KO SMC not only decreased intracellular Ca2+ spikes but also disrupted their coupling between cells resulting in decreased contractility. The role of SRF in the regulation of SMC phenotype and function provides new insight into how SMC lose their contractility leading to hypomotility in pathophysiological conditions within the GI tract. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC5289827?pdf=render; https://doaj.org/toc/1932-6203; https://doaj.org/article/e61d17fcddeb42c798c6f15cef0042fa |
| DOI: | 10.1371/journal.pone.0171262 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0171262 https://doaj.org/article/e61d17fcddeb42c798c6f15cef0042fa |
| رقم الانضمام: | edsbas.73B771B3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0171262 |