Academic Journal
Astrocyte ensheathment of calyx‐forming axons of the auditory brainstem precedes accelerated expression of myelin genes and myelination by oligodendrocytes
| العنوان: | Astrocyte ensheathment of calyx‐forming axons of the auditory brainstem precedes accelerated expression of myelin genes and myelination by oligodendrocytes |
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| المؤلفون: | Heller, Daniel T., Kolson, Douglas R., Brandebura, Ashley N., Amick, Emily M., Wan, Jun, Ramadan, Jad, Holcomb, Paul S., Liu, Sheng, Deerinck, Thomas J., Ellisman, Mark H., Qian, Jiang, Mathers, Peter H., Spirou, George A. |
| المساهمون: | National Institute of General Medical Sciences, National Institute on Deafness and Other Communication Disorders |
| المصدر: | Journal of Comparative Neurology ; volume 532, issue 2 ; ISSN 0021-9967 1096-9861 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2023 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Early postnatal brain development involves complex interactions among maturing neurons and glial cells that drive tissue organization. We previously analyzed gene expression in tissue from the mouse medial nucleus of the trapezoid body (MNTB) during the first postnatal week to study changes that surround rapid growth of the large calyx of Held (CH) nerve terminal. Here, we present genes that show significant changes in gene expression level during the second postnatal week, a developmental timeframe that brackets the onset of airborne sound stimulation and the early stages of myelination. Gene Ontology analysis revealed that many of these genes are related to the myelination process. Further investigation of these genes using a previously published cell type‐specific bulk RNA‐Seq data set in cortex and our own single‐cell RNA‐Seq data set in the MNTB revealed enrichment of these genes in the oligodendrocyte lineage (OL) cells. Combining the postnatal day (P)6–P14 microarray gene expression data with the previously published P0–P6 data provided fine temporal resolution to investigate the initiation and subsequent waves of gene expression related to OL cell maturation and the process of myelination. Many genes showed increasing expression levels between P2 and P6 in patterns that reflect OL cell maturation. Correspondingly, the first myelin proteins were detected by P4. Using a complementary, developmental series of electron microscopy 3D image volumes, we analyzed the temporal progression of axon wrapping and myelination in the MNTB. By employing a combination of established ultrastructural criteria to classify reconstructed early postnatal glial cells in the 3D volumes, we demonstrated for the first time that astrocytes within the mouse MNTB extensively wrap the axons of the growing CH terminal prior to OL cell wrapping and compaction of myelin. Our data revealed significant expression of several myelin genes and enrichment of multiple genes associated with lipid metabolism in astrocytes, which may ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/cne.25552 |
| الاتاحة: | http://dx.doi.org/10.1002/cne.25552 https://onlinelibrary.wiley.com/doi/pdf/10.1002/cne.25552 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.73B2F251 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/cne.25552 |
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