Academic Journal
Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations
| العنوان: | Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations |
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| المؤلفون: | Maric Irena, Viaggi Silvia, Caria Paola, Frau Daniela V, Degan Paolo, Vanni Roberta |
| المصدر: | Molecular Cytogenetics, Vol 4, Iss 1, p 26 (2011) |
| بيانات النشر: | BMC |
| سنة النشر: | 2011 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | thyroid carcinoma, centrosome, mitotic spindle, RET/PTC, BRAF, Genetics, QH426-470 |
| الوصف: | Background Differentiated thyroid carcinoma offers a good model to investigate the possible correlation between specific gene mutations and chromosome instability. Papillary thyroid neoplasms are characterized by different mutually exclusive genetic alterations, some of which are associated with aneuploidy and aggressive phenotype. Results We investigated the centrosome status and mitotic abnormalities in three thyroid carcinoma-derived cell lines, each maintaining the specific, biologically relevant gene alteration harbored by the parental tumors: RET/PTC1 rearrangement in TPC1; heterozygous and homozygous BRAF V600E mutation in K1 and in B-CPAP, respectively. B-CPAP cells showed a statistically significant ( P < 0.01) higher frequency of abnormal mitotic figures compared to TPC1 and K1 cells. Conclusions Our data indicate that RET/PTC1 oncogenic activity is not related to mitotic chromosome impairment and missegregation whereas, based on the consistent difference in types/frequencies of centrosome and spindle abnormalities observed between K1 and B-CPAP cells, the hetero/homozygous allelic status of BRAF V600E mutation seems to be not irrelevant in respect to chromosomal instability development. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1755-8166 |
| Relation: | http://www.molecularcytogenetics.org/content/4/1/26; https://doaj.org/toc/1755-8166; https://doaj.org/article/ed6494c7c04142cb8a400f7d47c48719 |
| DOI: | 10.1186/1755-8166-4-26 |
| الاتاحة: | https://doi.org/10.1186/1755-8166-4-26 https://doaj.org/article/ed6494c7c04142cb8a400f7d47c48719 |
| رقم الانضمام: | edsbas.73AB3135 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17558166 |
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| DOI: | 10.1186/1755-8166-4-26 |