Academic Journal
Detection, quantification, and characterization by whole-slide image analysis of bacterial infection by Vibrio aestuarianus, stained by immunohistochemistry, in the pacific oyster Magallana gigas
| العنوان: | Detection, quantification, and characterization by whole-slide image analysis of bacterial infection by Vibrio aestuarianus, stained by immunohistochemistry, in the pacific oyster Magallana gigas |
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| المؤلفون: | Geslin, Valentin, Tomasetti, Luca, Gulsrud, Thor Ole, Parizadeh, Leila, Travers, Marie-Agnes |
| المساهمون: | University of Stavanger, LIttoral ENvironnement et Sociétés (LIENSs), Institut national des sciences de l'Univers (INSU - CNRS)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010) |
| المصدر: | ISSN: 0044-8486. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2025 |
| المجموعة: | Institut national des sciences de l'Univers: HAL-INSU |
| مصطلحات موضوعية: | Digital histopathology, Immunohistochemistry, Magallana gigas, Vibrio aestuarianus, Whole slide image, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny |
| الوصف: | International audience ; The growth of marine bivalve aquaculture is presented as a potential measure to effectively provide seafood for human consumption while preserving wild populations. It can be an economic driver in coastal areas while also providing important ecosystem services such as filtration of phytoplankton and carbonate buffering. However, as with other farming practices, increased densities of individuals in a confined space often results in disease outbreaks. Pathogens developing under these conditions can easily spread among farmed stocks, affecting some or possibly most or all production lines, potentially causing adverse economic consequences on the local aquaculture sector, spreading over large areas and eventually affecting wild populations as well. Development and implementation of early detection methods for pathogen infection are imperative to maintain and expand aquaculture activities. In this context, a new method to quantify and characterize infection by Vibrio aestuarianus in the Pacific oyster, Magallana gigas, based on whole-slide image analysis of histological slides stained by immunohistochemistry is presented. The method is used to automatically measure the proportion of tissue infected by IHC-stained bacteria from each image and to characterize bacteria spread in the tissue. The pro portion of tissue infected by IHC-stained bacteria and spatial dispersion indexes, used to characterize 2D bac terial dispersion, were generally associated with the quantity of bacteria previously measured by quantitative polymerase chain reaction (qPCR). These results suggest a pattern of infection where V. aestuarianus tends to be more clustered and less randomly spread in the organism with increased infection. Advantages, limitations, and potential ways to improve the method are discussed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/j.aquaculture.2024.741983 |
| الاتاحة: | https://hal.science/hal-04829499 https://hal.science/hal-04829499v1/document https://hal.science/hal-04829499v1/file/Geslin-2025-Aquacult-Detection-Manuscrit.pdf https://doi.org/10.1016/j.aquaculture.2024.741983 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.735DECE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.aquaculture.2024.741983 |
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