Academic Journal
Time-lapse imaging of alveologenesis in mouse precision-cut lung slices
| العنوان: | Time-lapse imaging of alveologenesis in mouse precision-cut lung slices |
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| المؤلفون: | Akram, K, Yates, L, Mongey, R, Rothery, S, Gaboriau, D, Sanderson, J, Hind, M, Griffiths, M, Dean, C |
| المساهمون: | The Leverhulme Trust, Royal Brompton & Harefield NHS Foundation Trust |
| بيانات النشر: | Bio-Protocol |
| سنة النشر: | 2019 |
| المجموعة: | Imperial College London: Spiral |
| الوصف: | Alveoli are the gas-exchange units of lung. The process of alveolar development, alveologenesis, is regulated by a complex network of signaling pathways that act on various cell types including alveolar type I and II epithelial cells, fibroblasts and the vascular endothelium. Dysregulated alveologenesis results in bronchopulmonary dysplasia in neonates and in adults, disrupted alveolar regeneration is associated with chronic lung diseases including COPD and pulmonary fibrosis. Therefore, visualizing alveologenesis is critical to understand lung homeostasis and for the development of effective therapies for incurable lung diseases. We have developed a technique to visualize alveologenesis in real-time using a combination of widefield microscopy and image deconvolution of precision-cut lung slices. Here, we describe this live imaging technique in step-by-step detail. This time-lapse imaging technique can be used to capture the dynamics of individual cells within tissue slices over a long time period (up to 16 h), with minimal loss of fluorescence or cell toxicity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2331-8325 |
| Relation: | Bio-protocol; http://hdl.handle.net/10044/1/74160; https://doi.org/10.21769/bioprotoc.3403; RPG-2015-226; B1064 |
| DOI: | 10.21769/bioprotoc.3403 |
| الاتاحة: | http://hdl.handle.net/10044/1/74160 https://doi.org/10.21769/bioprotoc.3403 |
| Rights: | © 2019 Copyright Akram et al. This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/). |
| رقم الانضمام: | edsbas.734AC080 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23318325 |
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| DOI: | 10.21769/bioprotoc.3403 |