Academic Journal
Revised structure of the polysaccharide from Acinetobacter baumannii LUH5551 assigned as the K63 type capsular polysaccharide
| العنوان: | Revised structure of the polysaccharide from Acinetobacter baumannii LUH5551 assigned as the K63 type capsular polysaccharide |
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| المؤلفون: | Arbatsky, NP, Kasimova, AA, Shashkov, AS, Shneider, MM, Popova, AV, Perepelov, AV, Hall, RM, Kenyon, JJ, Knirel, YA |
| بيانات النشر: | Elsevier BV |
| سنة النشر: | 2024 |
| المجموعة: | Griffith University: Griffith Research Online |
| مصطلحات موضوعية: | Medical biochemistry - carbohydrates, Biochemistry and cell biology, Organic chemistry, Acinetobacter baumannii, Capsular polysaccharide, K locus, K63, LUH5551 |
| الوصف: | K63 capsular polysaccharide produced by Acinetobacter baumannii isolate LUH5551 (previously designated isolate O24) was re-examined using sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy. Though previously reported as O24 consisting of linear tetrasaccharide units that include a 7-acetamido-5-acylamino form of 8-epilegionaminic acid [8eLeg5R7Ac, acylated at C5 with (S)-3-hydroxybutanoyl or acetyl (1:1)], the elucidated structure of the K63 type capsule was found to include a derivative of 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid, Leg5Ac7R, where R is either (S)-3-hydroxybutanoyl or an acetyl group (∼1:1 ratio). This finding is consistent with the presence of the lgaABCHIFG gene module for Leg5Ac7R biosynthesis in the KL63 gene cluster at the capsular polysaccharide (CPS) biosynthesis K locus in the LUH5551 genome. The glycosyltransferases (Gtrs) and Wzy polymerase encoded by KL63 were assigned to linkages in the linear K63 tetrasaccharide unit and linkage of the K63 units. ; Full Text |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0008-6215 |
| Relation: | Carbohydrate Research; Arbatsky, NP; Kasimova, AA; Shashkov, AS; Shneider, MM; Popova, AV; Perepelov, AV; Hall, RM; Kenyon, JJ; Knirel, YA, Revised structure of the polysaccharide from Acinetobacter baumannii LUH5551 assigned as the K63 type capsular polysaccharide, Carbohydrate Research, 2024, 535, pp. 109020; http://hdl.handle.net/10072/430026 |
| DOI: | 10.1016/j.carres.2023.109020 |
| الاتاحة: | http://hdl.handle.net/10072/430026 https://doi.org/10.1016/j.carres.2023.109020 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ ; © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). ; open access |
| رقم الانضمام: | edsbas.7342145D |
| قاعدة البيانات: | BASE |
| تدمد: | 00086215 |
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| DOI: | 10.1016/j.carres.2023.109020 |