Academic Journal
Coexpression of SOX10/CD271 (p75(NTR)) and β-Galactosidase in large to giant congenital melanocytic nevi of pediatric patients
| العنوان: | Coexpression of SOX10/CD271 (p75(NTR)) and β-Galactosidase in large to giant congenital melanocytic nevi of pediatric patients |
|---|---|
| المؤلفون: | Barysch, Marjam J, Levesque, Mitchell P, Cheng, Phil, Karpova, Maria B, Mihic-Probst, Daniela, Civenni, Gianluca, Shakhova, Olga, Sommer, Lukas, Biedermann, Thomas, Schiestl, Clemens, Dummer, Reinhard |
| المصدر: | Barysch, Marjam J; Levesque, Mitchell P; Cheng, Phil; Karpova, Maria B; Mihic-Probst, Daniela; Civenni, Gianluca; Shakhova, Olga; Sommer, Lukas; Biedermann, Thomas; Schiestl, Clemens; Dummer, Reinhard (2014). Coexpression of SOX10/CD271 (p75(NTR)) and β-Galactosidase in large to giant congenital melanocytic nevi of pediatric patients. Dermatopathology, 1(1):35-46. |
| بيانات النشر: | Karger |
| سنة النشر: | 2014 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Institute of Anatomy, Clinic for Oncology and Hematology, Institute of Pathology and Molecular Pathology, Dermatology Clinic, 610 Medicine & health |
| الوصف: | BACKGROUND: Congenital melanocytic nevi (CMNs) are melanocytic neoplasms that can transform into melanoma. However, this development is impeded in the majority of cases and mostly affects patients with large or giant CMNs. METHODS: To elucidate mechanisms that keep CMNs from malignant transformation, CMN tissue biopsies were investigated for p-ERK and senescence markers by immunohistochemistry and for SOX10/CD271 (p75(NTR)) by immunofluorescence. CMN cells were cultivated, and MTT assays were performed for evaluating cell viability. Mutation status for NRAS and BRAF was performed by real-time PCR. RESULTS: 13 CMNs (from patients aged 0.5-11.8 years, mean: 2.7) showed immunoreactivity for SOX10/CD271 (p75(NTR)) in 34.2%. p-ERK was immunoreactive in 80% (4/5); β-galactosidase was significantly stronger expressed in CMNs compared to melanocytic nevi of patients over 70 years (p = 0.0085). The 5 CMN cultures were immunoreactive for SOX10/CD271 (p75(NTR)) in 36.7%. By silencing SOX10 by siRNA in 2 CMN cell cultures, cell viability decreased significantly. NRAS(Q61K) mutation was found in 91.7% (11/12) and BRAF(V600E) in 6.3% of all analyzable CMNs (1/16). CONCLUSIONS: Oncogene-induced senescence might prevent malignant transformation through activation of the mitogen-activated protein kinase pathway. SOX10 is necessary for the viability of human CMN cell cultures and may be responsible for clinical changes during aging. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2296-3529 |
| Relation: | https://www.zora.uzh.ch/id/eprint/131001/1/Coexpression_of_SOX10.pdf; info:pmid/27047921; urn:issn:2296-3529 |
| DOI: | 10.5167/uzh-131001 |
| DOI: | 10.1159/000362490 |
| الاتاحة: | https://www.zora.uzh.ch/id/eprint/131001/ https://www.zora.uzh.ch/id/eprint/131001/1/Coexpression_of_SOX10.pdf https://doi.org/10.5167/uzh-131001 https://doi.org/10.1159/000362490 |
| Rights: | info:eu-repo/semantics/openAccess ; Creative Commons: Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) ; http://creativecommons.org/licenses/by-nc/3.0/ |
| رقم الانضمام: | edsbas.72C29937 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22963529 |
|---|---|
| DOI: | 10.5167/uzh-131001 |