Academic Journal
Stanozolol induces ventricular dysfunction by decreasing phospholamban phosphorylation in heart tissue of LDLr-/- mice ; El estanozolol induce disfunción ventricular al reducir la fosforilación de fosfolamban en el tejido cardíaco de ratones LDLr-/- ; O estanozolol induz disfunção ventricular pela redução da fosforilação do fosfolambam no tecido cardíaco de camundongos LDLr-/-
| العنوان: | Stanozolol induces ventricular dysfunction by decreasing phospholamban phosphorylation in heart tissue of LDLr-/- mice ; El estanozolol induce disfunción ventricular al reducir la fosforilación de fosfolamban en el tejido cardíaco de ratones LDLr-/- ; O estanozolol induz disfunção ventricular pela redução da fosforilação do fosfolambam no tecido cardíaco de camundongos LDLr-/- |
|---|---|
| المؤلفون: | Andrade, Tadeu Uggere de, Dubois Fillho, Dionisio, Silva, Cristiane Lyrio da, Silva, Mirian de Almeida, Almeida, Simone Alves de, Nascimento, Andrews Marques do, Bissoli, Nazaré Souza, Brasil, Girlandia Alexandre, Lima, Ewelyne Miranda de |
| المصدر: | Research, Society and Development; Vol. 11 No. 5; e12911527876 ; Research, Society and Development; Vol. 11 Núm. 5; e12911527876 ; Research, Society and Development; v. 11 n. 5; e12911527876 ; 2525-3409 |
| بيانات النشر: | Research, Society and Development |
| سنة النشر: | 2022 |
| المجموعة: | Research, Society and Development (E-Journal) |
| مصطلحات موضوعية: | Anabolic androgenic steroids, Hemodynamic parameters, Cardiac remodeling, Phospholamban phosphorylation, Esteróide anabolico androgênico, Parâmetros hemodinâmicos, Remodelamento cardíaco, Fosfolambam fosforilada, Esteroide androgénico anabólico, Parámetros hemodinâmicos, Remodelado cardíaco, Fosfolambano fosforilado |
| الوصف: | Stanozolol is a steroid that causes lipid deposition in LDLr-/- mice, although the mechanism by which this dyslipidemia results in cardiac dysfunction is little understood. The aim of this study was to evaluate the effect of stanozolol on cardiac contractility and the participation of myocardial phospholamban (pPLB) phosphorylation in an atherosclerosis mouse model. LDL receptor knockout mice (LDLr-/-) were fed a standard chow diet and received weekly subcutaneous injections of either saline (control, C group) or 20 mg/kg stanozolol (S group). After 8 weeks, hemodynamic parameters were assessed in the left ventricle. The heart was collected, weighted for hypertrophy evaluation, and kept in formalin buffer for morphometric analysis (H&E) and collagen quantification (Picrossirius). Protein expression of PLB and its phosphorylated form (p-PLB) in the left ventricle was determined by western blot. We observed that stanozolol treatment favored cardiac hypertrophy and collagen deposition in heart tissue. Also, stanozolol induced left ventricle dysfunction, increasing PBL expression and decreasing the p-PLB/PLB ratio. Altogether, our data showed that stanozolol causes cardiac remodeling and ventricular dysfunction by decreasing PLB phosphorylation in the left ventricle of LDLr-/- mice. ; El estanozolol es un esteroide que promueve el depósito de lípidos en las arterias de ratones LDLr-/-, sin embargo, el mecanismo por el cual la dislipidemia promueve la disfunción cardíaca en estos animales aún no se conoce bien. Por lo tanto, el objetivo del presente estudio es evaluar el efecto del estanozolol sobre la contractilidad cardíaca y la participación de la fosforilación de la proteína fosfolambano miocárdico (pPBL) en el modelo animal de aterosclerosis. Se alimentó a ratones sin receptor de LDL (LDLr-/-) con una dieta estándar de casa de animales y recibieron inyecciones subcutáneas semanales de solución salina (grupo de control, C) o 20 mg/kg de estanozolol (grupo S). Después de ocho semanas de tratamiento, se ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://rsdjournal.org/index.php/rsd/article/view/27876/24396; https://rsdjournal.org/index.php/rsd/article/view/27876 |
| DOI: | 10.33448/rsd-v11i5.27876 |
| الاتاحة: | https://rsdjournal.org/index.php/rsd/article/view/27876 https://doi.org/10.33448/rsd-v11i5.27876 |
| Rights: | Copyright (c) 2022 Tadeu Uggere de Andrade; Dionisio Dubois Fillho; Cristiane Lyrio da Silva; Mirian de Almeida Silva; Simone Alves de Almeida; Andrews Marques do Nascimento; Nazaré Souza Bissoli; Girlandia Alexandre Brasil; Ewelyne Miranda de Lima ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.72AA9CC9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.33448/rsd-v11i5.27876 |
|---|