Academic Journal
Secretion of biologically active pancreatitis-associated protein I ( PAP) by genetically modified dairy Lactococcus lactis NZ9000 in the prevention of intestinal mucositis
| العنوان: | Secretion of biologically active pancreatitis-associated protein I ( PAP) by genetically modified dairy Lactococcus lactis NZ9000 in the prevention of intestinal mucositis |
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| المؤلفون: | Carvalho, Rodrigo D., Martins Breyner, Natalia, Menezes-Garcia, Zelia, Rodrigues, Nubia M., Lemos, Luisa, Maioli, Tatiane U., Souza, Danielle da Gloria, Carmona, Denise, de Faria, Ana M. C., Langella, Philippe, Chatel, Jean-Marc, Bermudez-Humaran, Luis G., Figueiredo, Henrique C. P., Azevedo, Vasco, de Azevedo, Marcela S. |
| المساهمون: | Instituto de Ciencias Biologicas Minas Gerais (ICB), Universidade Federal de Minas Gerais = Federal University of Minas Gerais Belo Horizonte, Brazil (UFMG), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, AgroParisTech, Coordination for the Improvement of Higher Education Personnel (CAPES), National Counsel of Technological and Scientific Development (CNPq) |
| المصدر: | ISSN: 1475-2859 ; Microbial Cell Factories ; https://hal.science/hal-01607391 ; Microbial Cell Factories, 2017, 16, pp.1-11. ⟨10.1186/s12934-017-0624-x⟩. |
| بيانات النشر: | CCSD BioMed Central |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Pancreatitis-associated protein, Mucositis, Lactococcus lactis, 5-Fluoracil, [SDV]Life Sciences [q-bio] |
| الوصف: | Background: Mucositis is one of the most relevant gastrointestinal inflammatory conditions in humans, generated by the use of chemotherapy drugs, such as 5-fluoracil (5-FU). 5-FU-induced mucositis affects 80% of patients undergoing oncological treatment causing mucosal gut dysfunctions and great discomfort. As current therapy drugs presents limitations in alleviating mucositis symptoms, alternative strategies are being pursued. Recent studies have shown that the antimicrobial pancreatitis-associated protein (PAP) has a protective role in intestinal inflammatory processes. Indeed, it was demonstrated that a recombinant strain of Lactococcus lactis expressing human PAP (LL-PAP) could prevent and improve murine DNBS-induced colitis, an inflammatory bowel disease (IBD) that causes severe inflammation of the colon. Hence, in this study we sought to evaluate the protective effects of LL-PAP on 5-FU-induced experimental mucositis in BALB/c mice as a novel approach to treat the disease. Results: Our results show that non-recombinant L. lactis NZ9000 have antagonistic activity, in vitro, against the enteroinvasive gastrointestinal pathogen L. monocytogenes and confirmed PAP inhibitory effect against Opportunistic E. faecalis. Moreover, L. lactis was able to prevent histological damage, reduce neutrophil and eosinophil infiltration and secretory Immunoglobulin-A in mice injected with 5-FU. Recombinant lactococci carrying antimicrobial PAP did not improve those markers of inflammation, although its expression was associated with villous architecture preservation and increased secretory granules density inside Paneth cells in response to 5-FU inflammation. Conclusions: We have demonstrated for the first time that L. lactis NZ9000 by itself, is able to prevent 5-FU-induced intestinal inflammation in BALB/c mice. Moreover, PAP delivered by recombinant L. lactis strain showed additional protective effects in mice epithelium, revealing to be a promising strategy to treat intestinal mucositis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28193209; PRODINRA: 394291; PUBMED: 28193209; WOS: 000397728000001 |
| DOI: | 10.1186/s12934-017-0624-x |
| الاتاحة: | https://hal.science/hal-01607391 https://hal.science/hal-01607391v1/document https://hal.science/hal-01607391v1/file/2017_Carvalho_Microbial%20Cell%20Factories_1.pdf https://doi.org/10.1186/s12934-017-0624-x |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.728E685E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12934-017-0624-x |
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