Academic Journal
Potentiation of radiotherapy by a localized antiangiogenic gene therapy.
| العنوان: | Potentiation of radiotherapy by a localized antiangiogenic gene therapy. |
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| المؤلفون: | Crokart, Nathalie, Danhier, Fabienne, Daugimont, Liévin, Gonçalves, Nelio, Jordan, Bénédicte, Grégoire, Vincent, Feron, Olivier, Bouquet, Céline, Gallez, Bernard, Préat, Véronique |
| المساهمون: | UCL - SSS/LDRI - Louvain Drug Research Institute, UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie, UCL - (SLuc) Service de radiothérapie oncologique, UCL - (SLuc) Service de médecine nucléaire |
| المصدر: | Radiotherapy & Oncology, Vol. 107, no. 2, p. 252-258 (2013) |
| بيانات النشر: | Elsevier Ireland Ltd |
| سنة النشر: | 2013 |
| المجموعة: | DIAL@USL-B (Université Saint-Louis, Bruxelles) |
| مصطلحات موضوعية: | Antiangiogenesis, Gene therapy, Radiosensitization, Cancer, Electrotransfer, Tumor microenvironment |
| الوصف: | BACKGROUND AND PURPOSE: We hypothesized that electrotransfer of a plasmid encoding an antiangiogenic factor, the recombinant disintegrin domain of ADAM-15, (pRDD) could modify the tumor microenvironment and radiosensitize tumor. MATERIALS AND METHODS: pRDD was injected in the TLT tumor or FSaII fibrosarcomas before electroporation. pO2 in tumors and oxygen consumption in vitro were measured by electronic paramagnetic resonance (EPR) oximetry. Tumor perfusion was assessed by laser doppler imaging and patent blue assay. RESULTS: pRDD electrotransfer caused a significant delay in TLT growth and an anti-angiogenic effect. It significantly increased tumor pO2 in TLT and FSaII for at least 4days. pRDD electrotransfer and radiotherapy were more effective than either treatment alone. Modifications of tumor microenvironment were evaluated: tumor perfusion and interstitial fluid pressure were not modified. Oxygen consumption by the cells was decreased resulting both from a decrease in oxygen consumption rate and from a decrease in cell viability. CONCLUSION: The combination of localized antiangiogenic gene therapy and radiotherapy applied in the time of maximal oxygenation could be a promising alternative for cancer treatment. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0167-8140 1879-0887 |
| Relation: | boreal:128204; http://hdl.handle.net/2078.1/128204; info:pmid/23623728; urn:ISSN:0167-8140; urn:EISSN:1879-0887 |
| DOI: | 10.1016/j.radonc.2013.03.018 |
| الاتاحة: | http://hdl.handle.net/2078.1/128204 https://doi.org/10.1016/j.radonc.2013.03.018 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.72357348 |
| قاعدة البيانات: | BASE |
| تدمد: | 01678140 18790887 |
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| DOI: | 10.1016/j.radonc.2013.03.018 |