Academic Journal
Fatty Acid Binding Protein 6 Inhibition Decreases Cell Cycle Progression, Migration and Autophagy in Bladder Cancers
| العنوان: | Fatty Acid Binding Protein 6 Inhibition Decreases Cell Cycle Progression, Migration and Autophagy in Bladder Cancers |
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| المؤلفون: | Chieh-Hsin Lin, Hsin-Han Chang, Chien-Rui Lai, Hisao-Hsien Wang, Wen-Chiuan Tsai, Yu-Ling Tsai, Chih-Ying Changchien, Yu-Chen Cheng, Sheng-Tang Wu, Ying Chen |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 2154, p 2154 (2022) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | FABP6, bladder cancer, proliferation, cell cycle, migration, autophagy, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Bladder cancer (BC) has a high recurrence rate worldwide. The aim of this study was to evaluate the role of fatty acid binding protein 6 (FABP6) in proliferation and migration in human bladder cancer cells. Cell growth was confirmed by MTT and colony formation assay. Western blotting was used to explore protein expressions. Wound healing and Transwell assays were performed to evaluate the migration ability. A xenograft animal model with subcutaneous implantation of BC cells was generated to confirm the tumor progression. Knockdown of FABP6 reduced cell growth in low-grade TSGH-8301 and high-grade T24 cells. Cell cycle blockade was observed with the decrease of CDK2, CDK4, and Ki67 levels in FABP6-knockdown BC cells. Interestingly, knockdown of FBAP6 led to downregulation of autophagic markers and activation of AKT-mTOR signaling. The application of PI3K/AKT inhibitor decreased cell viability mediated by FABP6-knockdown additionally. Moreover, FABP6-knockdown reduced peroxisome proliferator-activated receptor γ and retinoid X receptor α levels but increased p-p65 expression. Knockdown of FABP6 also inhibited BC cell motility with focal adhesive complex reduction. Finally, shFABP6 combined with cisplatin suppressed tumor growth in vivo. These results provide evidence that FABP6 may be a potential target in BC cells progression. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/4/2154; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/7cb9e0a7271e487398c83d1acd01dc2b |
| DOI: | 10.3390/ijms23042154 |
| الاتاحة: | https://doi.org/10.3390/ijms23042154 https://doaj.org/article/7cb9e0a7271e487398c83d1acd01dc2b |
| رقم الانضمام: | edsbas.72327D37 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23042154 |