Academic Journal

Anionic Phospholipids Stimulate the Proton Pumping Activity of the Plant Plasma Membrane P-Type H+-ATPase

التفاصيل البيبلوغرافية
العنوان: Anionic Phospholipids Stimulate the Proton Pumping Activity of the Plant Plasma Membrane P-Type H+-ATPase
المؤلفون: Laura C. Paweletz, Simon L. Holtbrügge, Malina Löb, Dario De Vecchis, Lars V. Schäfer, Thomas Günther Pomorski, Bo Højen Justesen
المصدر: International Journal of Molecular Sciences; Volume 24; Issue 17; Pages: 13106
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2023
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: H + -ATPase, lipid–protein interaction, liposome, molecular modeling, proton pump, reconstitution
جغرافية الموضوع: agris
الوصف: The activity of membrane proteins depends strongly on the surrounding lipid environment. Here, we characterize the lipid stimulation of the plant plasma membrane H+-ATPase Arabidopsis thaliana H+-ATPase isoform 2 (AHA2) upon purification and reconstitution into liposomes of defined lipid compositions. We show that the proton pumping activity of AHA2 is stimulated by anionic phospholipids, especially by phosphatidylserine. This activation was independent of the cytoplasmic C-terminal regulatory domain of the pump. Molecular dynamics simulations revealed several preferential contact sites for anionic phospholipids in the transmembrane domain of AHA2. These contact sites are partially conserved in functionally different P-type ATPases from different organisms, suggesting a general regulation mechanism by the membrane lipid environment. Our findings highlight the fact that anionic lipids play an important role in the control of H+-ATPase activity.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
Relation: Molecular Biology; https://dx.doi.org/10.3390/ijms241713106
DOI: 10.3390/ijms241713106
الاتاحة: https://doi.org/10.3390/ijms241713106
Rights: https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.71E512DC
قاعدة البيانات: BASE