Academic Journal
C ° 2000 Kluwer Academic Publishers Bcl-2 protects against apoptosis-related microtubule alterations in neuronal cells
| العنوان: | C ° 2000 Kluwer Academic Publishers Bcl-2 protects against apoptosis-related microtubule alterations in neuronal cells |
|---|---|
| المؤلفون: | Rony Nuydens, Gwenda Dispersyn, Gerd Van, Den Kieboom, Mirjam De Jong, Rich Connors, Frans Ramaekers, Marcel Borgers, Hugo Geerts |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://arno.unimaas.nl/show.cgi?fid=3797. |
| سنة النشر: | 1999 |
| المجموعة: | CiteSeerX |
| مصطلحات موضوعية: | Apoptosis, bcl-2, microtubules, neurites, tau, taxol |
| الوصف: | Bcl-2 is a gene with clear anti-apoptotic properties in neu-rodegenerative conditions. One of the earliest hallmarks of degeneration in neuronal cell cultures is the loss of neurite morphology. Therefore the effect of Bcl-2 on neu-ronal morphology and microtubule stability was studied in nerve growth factor differentiated PC12 cells. Micro-tubule dynamics were modulated using the microtubule stabilizer taxol and the microtubule destabilizer, okadaic acid, a protein phosphatase inhibitor. It was shown that Bcl-2 protects against both taxol- and okadaic acid induced neurite retraction. Bcl-2 overexpression also sig-nificantly reduced the increased ratio of acetylated tubu-lin over total tubulin induced by taxol treatment. Inter-estingly, Bcl-2 attenuates the decrease of the same ratio after exposure to okadaic acid, suggesting that Bcl-2 is able to normalize the level of acetylated tubulin. In ad-dition, cell death and nuclear fragmentation, induced by okadaic acid, were reduced in Bcl-2 overexpressing cells. This protection is either downstream or independent of tau phosphorylation as quantitative immunocytochem-istry with AT8 showed that Bcl-2 did not modify the level of tau phosphorylation. The data suggest that the protective effect of Bcl-2 on the neuronal cytoskeleton is probably linked to changes in the post-translational modification of tubulin. |
| نوع الوثيقة: | text |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.516.3616; http://arno.unimaas.nl/show.cgi?fid=3797 |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.516.3616 http://arno.unimaas.nl/show.cgi?fid=3797 |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.71CA4903 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |