Academic Journal
Rapid molecular detection of pathogenic microorganisms and antimicrobial resistance markers in blood cultures: evaluation and utility of the next-generation FilmArray Blood Culture Identification 2 panel
| العنوان: | Rapid molecular detection of pathogenic microorganisms and antimicrobial resistance markers in blood cultures: evaluation and utility of the next-generation FilmArray Blood Culture Identification 2 panel |
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| المؤلفون: | Holma, Tanja, Torvikoski, Jukka, Friberg, Nathalie, Nevalainen, Annika, Tarkka, Eveliina, Antikainen, Jenni, Martelin, Jari J. |
| المساهمون: | University of Helsinki including Helsinki University Central Hospital |
| المصدر: | European Journal of Clinical Microbiology & Infectious Diseases ; volume 41, issue 3, page 363-371 ; ISSN 0934-9723 1435-4373 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2021 |
| الوصف: | Rapid detection of pathogens causing bloodstream infections (BSI) directly from positive blood cultures is of highest importance in order to enable an adequate and timely antimicrobial therapy. In this study, the utility and performance of a recently launched next-generation fully automated test system, the Biofire FilmArray® Blood Culture Identification 2 (BCID2) panel, was evaluated using a set of 103 well-characterized microbial isolates including 29 antimicrobial resistance genes and 80 signal-positive and 23 signal-negative clinical blood culture samples. The results were compared to culture-based reference methods, MALDI-TOF, and/or 16S rDNA sequencing. Of the clinical blood culture samples, 68 were monomicrobial (85.0%) and 12 polymicrobial (15.0%). Six samples contained ESBL ( bla CTX-M ), two MRSA ( mec A), and three MRSE ( mec A) isolates. In overall, the FilmArray BCID2 panel detected well on-panel targets and resistance markers from mono- and polymicrobial samples. However, one Klebsiella aerogenes and one Bacteroides ovatus were undetected, and the assay falsely reported one Shigella flexneri as Escherichia coli . Hence, the sensitivity and specificity for detecting microbial species were 98.8% (95%CI, 95.8–99.9%) and 99.9% (95%CI, 99.8–99.9%), respectively. The sensitivity and specificity for detecting of resistance gene markers were 100%. The results were available within 70 min from signal-positive blood cultures with minimal hands-on time. In conclusion, the BCID2 test allows reliable and simplified detection of a vast variety of clinically relevant microbes causing BSI and the most common antimicrobial resistance markers present among these isolates. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1007/s10096-021-04314-2 |
| DOI: | 10.1007/s10096-021-04314-2.pdf |
| DOI: | 10.1007/s10096-021-04314-2/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1007/s10096-021-04314-2 https://link.springer.com/content/pdf/10.1007/s10096-021-04314-2.pdf https://link.springer.com/article/10.1007/s10096-021-04314-2/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.71C95BCB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s10096-021-04314-2 |
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