Academic Journal
Characteristics of hprt‐mutant t cell lines in a lupus patient treated with cyclophosphamide
| العنوان: | Characteristics of hprt‐mutant t cell lines in a lupus patient treated with cyclophosphamide |
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| المؤلفون: | Wood, Geryl M., Dawisha, Sahar, Gourley, Mark |
| المصدر: | Arthritis & Rheumatism ; volume 37, issue 10, page 1548-1552 ; ISSN 0004-3591 1529-0131 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1994 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | This report describes T cell lines derived from a patient with subacute cutaneous lupus after treatment with intravenous pulse cyclophosphamide. We selected for mitotically active, hypoxanthine‐guanine phosphoribosyltransferase—deficient (HPRT—) T cells, by culture in a selective medium containing 6‐thioguanine. When HPRT— cell lines were derived 6 days after pulse cyclophosphamide (CYC) treatment, they were predominantly CD8+ and T cell receptor (TCR) γ/δ+, producing interferon‐γ (IFNγ). Cell lines derived 21 days after CYC treatment were CD4+, TCRα/β+ and produced both IFNγ and interleukin‐4. These results support a possible role for γ/δ+ T cells in subacute cutaneous lupus and suggest a mechanism for the therapeutic effect of CYC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/art.1780371021 |
| الاتاحة: | http://dx.doi.org/10.1002/art.1780371021 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fart.1780371021 https://onlinelibrary.wiley.com/doi/pdf/10.1002/art.1780371021 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.716B85C1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/art.1780371021 |
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