Academic Journal
The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells
| العنوان: | The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells |
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| المؤلفون: | Hayashi, M., Inagaki, Akihiro, Novak, I., Matsuda, H. |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2021 |
| المجموعة: | Tokushima University Institutional Repository / 徳島大学機関リポジトリ |
| مصطلحات موضوعية: | Adenosine receptor, CFTR, Cl− channel, Duct, Pancreas |
| الوصف: | Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, adenosine regulates transepithelial anion secretion in duct cells and is considered to play a role in acini-to-duct signaling. To identify the functional adenosine receptors and Cl− channels important for anion secretion, we herein performed experiments on Capan-1, a human pancreatic duct cell line, using open-circuit Ussing chamber and gramicidin-perforated patch-clamp techniques. The luminal addition of adenosine increased the negative transepithelial potential difference (V te) in Capan-1 monolayers with a half-maximal effective concentration value of approximately 10 μM, which corresponded to the value obtained on whole-cell Cl− currents in Capan-1 single cells. The effects of adenosine on V te, an equivalent short-circuit current (I sc), and whole-cell Cl− currents were inhibited by CFTRinh-172, a cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel inhibitor. The adenosine A2B receptor agonist, BAY 60-6583, increased I sc and whole-cell Cl− currents through CFTR Cl− channels, whereas the A2A receptor agonist, CGS 21680, had negligible effects. The A2B receptor antagonist, PSB 603, inhibited the response of I sc to adenosine. Immunohistochemical analysis showed that the A2A and A2B receptors colocalized with Ezrin in the luminal membranes of Capan-1 monolayers and in rat pancreatic ducts. Adenosine elicited the whole-cell Cl− currents in guinea pig duct cells. These results demonstrate that luminal adenosine regulates anion secretion by activating CFTR Cl− channels via adenosine A2B receptors on the luminal membranes of Capan-1 cells. The present study endorses that purinergic signaling is important in the regulation of pancreatic secretion. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 00316768 14322013 |
| Relation: | http://repo.lib.tokushima-u.ac.jp/files/public/11/115683/20210210141750346356/paejp_468_7_1171.pdf; AA00771833; http://repo.lib.tokushima-u.ac.jp/115683 |
| الاتاحة: | http://repo.lib.tokushima-u.ac.jp/115683 |
| Rights: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| رقم الانضمام: | edsbas.70F59775 |
| قاعدة البيانات: | BASE |
| تدمد: | 00316768 14322013 |
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