Academic Journal
Characterization of the immune system of Ellegaard Göttingen Minipigs - An important large animal model in experimental medicine
| العنوان: | Characterization of the immune system of Ellegaard Göttingen Minipigs - An important large animal model in experimental medicine |
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| المؤلفون: | Pernold, Clara P. S. (University of Veterinary Medicine Vienna), Saalmüller, Armin (University of Veterinary Medicine Vienna), Mair, Kerstin H. (University of Veterinary Medicine Vienna), Stadler, Maria (University of Veterinary Medicine Vienna), Lagumdzic, Emil (University of Veterinary Medicine Vienna), Sawyer, Spencer (University of Veterinary Medicine Vienna), Martin, Vera (University of Veterinary Medicine Vienna), Kreutzmann, Heinrich (University of Veterinary Medicine Vienna), Dürlinger, Sophie (University of Veterinary Medicine Vienna), Knecht, Christian (University of Veterinary Medicine Vienna), Ladinig, Andrea (University of Veterinary Medicine Vienna), Schmitt, Michael W (Merck KGaA), Jäckel, Sven (Merck KGaA) |
| المصدر: | Frontiers in Immunology 13 (2022) |
| بيانات النشر: | Frontiers Media Sa |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Delta T-Cells, Monoclonal-Antibodies, Natural-Killer, Lymphocyte Subsets, Reference Range, Cytotoxic-T, Porcine Nk, Expression, Blood, Swine |
| الوصف: | Interest in Ellegaard Göttingen Minipigs (EGMs) as a model in experimental medicine is continuously growing. The aim of this project is to increase the knowledge of the immune system of EGMs as information is still scarce. Therefore, we studied the postnatal maturation of their immune system from birth until 126 weeks of age. For the first 26 weeks of the study, animals were kept under pathogen-reduced conditions (SPF) and afterwards under conventional housing conditions. The development of the immune system was analyzed by monitoring changes in total numbers of leukocytes and lymphocytes of ten individuals and the composition of leukocyte populations by multi-color flow cytometry (FCM). We followed the presence of monocytes using monoclonal antibodies (mAbs) against CD172a+ and CD163+ and B cells based on the expression of CD79a. NK cells were distinguished as CD3-CD16+CD8α+/dim cells and further subdivided using NKp46 (CD335) expression into NKp46-, NKp46+, and NKp46high NK cells. T-cell receptor (TCR) γδ T cells were defined by the expression of TCR-γδ and different subsets were determined by their CD2 and perforin expression. TCR-αβ T cells were classified by their CD8β+ or CD4 expression. For monitoring their differentiation, expression of CD27 and perforin was investigated for CD8β++ T cells and CD8α together with CD27 for CD4+ T cells. We clearly detected a postnatal development of immune cell composition and identified phenotypes indicative of differentiation within the respective leukocyte subsets. Examination of the development of the antigen-specific immune system after transfer to different distinct housing conditions and after vaccination against common porcine pathogens such as porcine circovirus 2 (PCV2) revealed a markedly increased presence of more differentiated CD8+ and CD4+ T cells with central and effector memory T-cell phenotypes. To complement the findings, a PCV2 vaccine-specific antigen was used for in vitro restimulation experiments. We demonstrated antigen-specific proliferation of ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | isPartOf:https://phaidra.vetmeduni.ac.at/o:605[Open Access Publications]; https://phaidra.vetmeduni.ac.at/o:1719 |
| DOI: | 10.3389/fimmu.2022.1003986 |
| الاتاحة: | https://doi.org/10.3389/fimmu.2022.1003986 https://phaidra.vetmeduni.ac.at/o:1719 |
| Rights: | CC BY 4.0 International ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.70E930EA |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2022.1003986 |
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