التفاصيل البيبلوغرافية
| العنوان: |
Molecular Characterization of Gene 16s Rrna Micro Symbionts |
| المؤلفون: |
Marzuki, I. (Ismail), Noor, A. (Alfian), Nafie, N. L. (Nursiah), Djide, M. N. (M) |
| المصدر: |
Jurnal Administrasi dan Kebijakan Kesehatan Indonesia |
| بيانات النشر: |
Hasanuddin University |
| سنة النشر: |
2015 |
| المجموعة: |
neliti (Indonesia's Think Tank Database) |
| مصطلحات موضوعية: |
Indonesia, gene Molecules, characterization, micro-symbionts, Callyspongia sp |
| الوصف: |
Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://www.neliti.com/publications/109231/molecular-characterization-of-gene-16s-rrna-micro-symbionts |
| الاتاحة: |
https://www.neliti.com/publications/109231/molecular-characterization-of-gene-16s-rrna-micro-symbionts |
| Rights: |
(c) Jurnal Administrasi dan Kebijakan Kesehatan Indonesia, 2015 |
| رقم الانضمام: |
edsbas.7088D0E2 |
| قاعدة البيانات: |
BASE |