Academic Journal
Use of different carbon sources in cultivation of baker's yeast for production of glycerol-3-phosphate dehydrogenase
| العنوان: | Use of different carbon sources in cultivation of baker's yeast for production of glycerol-3-phosphate dehydrogenase |
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| المؤلفون: | Gattas, Edwil Aparecida de Lucca, Peres, Maristela F.S. |
| المساهمون: | Universidade Estadual Paulista (UNESP) |
| سنة النشر: | 2013 |
| المجموعة: | Universidade Estadual Paulista São Paulo: Repositório Institucional UNESP |
| مصطلحات موضوعية: | Baker's yeast, Glycerol-3-phosphate dehydrogenase, Induction, alcohol, cell extract, galactose, glucose, glycerol, glycerol 3 phosphate dehydrogenase, biomass, carbon source, cell culture, culture medium, enzyme assay, enzyme synthesis, fungus growth, genetic regulation, inoculation, metabolic regulation, nonhuman, pH, protein determination, Saccharomyces cerevisiae |
| الوصف: | PADC/FCF ; Fundação para o Desenvolvimento da UNESP (FUNDUNESP) ; The physiological state of yeast cells changes during culture growth as a consequence of environmental changes (nutrient limitations, pH and metabolic products). Cultures that grow exponentially are heterogeneous cell populations made up of cells regulated by different metabolic and/or genetic control systems. The strain of baker's yeast selected by plating commercial compressed yeast was used for the production of glycerol-3- phosphate dehydrogenase. Glycerol-3-phosphate dehydrogenase (GPD) has been widely used in the enzyme assays with diverse compounds of industrial interest, such as glycerol or glycerol phosphate, as well as a number of important bioanalytical applications. Each cell state determines the level of key enzymes (genetic control), fluxes through metabolic pathways (metabolic control), cell morphology and size. The present study was carried out to determine the effects of environmental conditions and carbon source on GPD production from baker's yeast. Glucose, glycerol, galactose and ethanol were used as carbon sources. Glycerol and ethanol assimilations required agitation, which was dependent on the medium volume in the fermentation flask for the greatest accumulation of intracellular GPD. Enzyme synthesis was also affected by the initial pH of the medium and inoculum size. The fermentation time required for a high level of enzyme formation decreased with the inoculum size. The greatest amount of enzyme (0.45 U/ml) was obtained with an initial pH of 4.5 in the medium containing ethanol or glycerol. The final pH was maintained in YP-ethanol, but in the YP-glycerol the final pH increased to 6.9 during growth. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 246-249 |
| اللغة: | English |
| ردمك: | 978-4-00-659861-7 4-00-659861-0 |
| تدمد: | 1459-0255 1459-0263 |
| Relation: | Journal of Food, Agriculture and Environment; 0,197; http://world-food.net/use-of-different-carbon-sources-in-cultivation-of-bakers-yeast-for-production-of-glycerol-3-phosphate-dehydrogenase/; Journal of Food, Agriculture and Environment, v. 11, n. 1, p. 246-249, 2013.; http://hdl.handle.net/11449/74811; 2-s2.0-84874526040; 4006598610021833 |
| الاتاحة: | http://hdl.handle.net/11449/74811 http://world-food.net/use-of-different-carbon-sources-in-cultivation-of-bakers-yeast-for-production-of-glycerol-3-phosphate-dehydrogenase/ |
| Rights: | closedAccess |
| رقم الانضمام: | edsbas.707791FD |
| قاعدة البيانات: | BASE |
| ردمك: | 9784006598617 4006598610 |
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| تدمد: | 14590255 14590263 |