التفاصيل البيبلوغرافية
| العنوان: |
Validation of a Simple HPLC-Based Method for Lysine Quantification for Ruminant Nutrition |
| المؤلفون: |
João Albuquerque, Susana Casal, Rebeca Cruz, Ingrid Van Dorpe, Margarida Rosa Garcez Maia, António José Mira Fonseca, Ana Rita Jordão Cabrita, Ana Rute Neves, Salette Reis |
| المصدر: |
Molecules; Volume 26; Issue 14; Pages: 4173 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
high-performance liquid chromatography, lysine quantification, biological samples |
| جغرافية الموضوع: |
agris |
| الوصف: |
Robust and selective quantification methods are required to better analyze feed supplementation effectiveness with specific amino acids. In this work, a reversed-phase high-performance liquid chromatography method with fluorescence detection is proposed and validated for lysine quantification, one of the most limiting amino acids in ruminant nutrition and essential towards milk production. To assess and widen method applicability, different matrices were considered: namely Li2CO3 buffer (the chosen standard reaction buffer), phosphate buffer solution (to mimic media in cellular studies), and rumen inoculum. The method was validated for all three matrices and found to be selective, accurate (92% ± 2%), and precise at both the inter- and intra-day levels in concentrations up to 225 µM, with detection and quantification limits lower than 1.24 and 4.14 µM, respectively. Sample stability was evaluated when stored at room temperature, 4 °C, and −20 °C, showing consistency for up to 48 h regardless of the matrix. Finally, the developed method was applied in the quantification of lysine on real samples. The results presented indicate that the proposed method can be applied towards free lysine quantification in ruminant feeding studies and potentially be of great benefit to dairy cow nutrition supplementation and optimization. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Analytical Chemistry; https://dx.doi.org/10.3390/molecules26144173 |
| DOI: |
10.3390/molecules26144173 |
| الاتاحة: |
https://doi.org/10.3390/molecules26144173 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.705638CB |
| قاعدة البيانات: |
BASE |