Academic Journal
Susceptibility of Clinical Enterobacterales Isolates With Common and Rare Carbapenemases to Mecillinam
| العنوان: | Susceptibility of Clinical Enterobacterales Isolates With Common and Rare Carbapenemases to Mecillinam |
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| المؤلفون: | Fuchs, Frieder, Ahmadzada, Aysel, Plambeck, Lars, Wille, Thorsten, Hamprecht, Axel |
| المساهمون: | DZIF, University of Cologne |
| المصدر: | Frontiers in Microbiology ; volume 11 ; ISSN 1664-302X |
| بيانات النشر: | Frontiers Media SA |
| سنة النشر: | 2021 |
| المجموعة: | Frontiers (Publisher - via CrossRef) |
| الوصف: | Purpose : To investigate the susceptibility of carbapenemase-producing Enterobacterales (CPE) to mecillinam based on the recently updated European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints for uncomplicated Urinary Tract Infection (uUTI). Methods : The challenge collection consisted of 105 molecularly characterized Enterobacterales [ Klebsiella spp. ( N = 49), Escherichia coli ( N = 30), Enterobacter cloacae ( n = 13), Citrobacter freundii ( N = 9), Proteus mirabilis ( N = 3), and Raoultella ornithinolytica ( N = 1)]. Isolates produced OXA-48 ( N = 18), OXA-48-like ( N = 18), VIM ( N = 22), NDM ( N = 22), KPC ( N = 12), IMI ( N = 9), IMP ( N = 6), GES ( N = 1), OXA-58 ( N = 2) or combinations thereof ( N = 5). MICs of carbapenems were determined by agar gradient diffusion (AGD). MICs of mecillinam were assessed by agar dilution (reference method) and compared to disk diffusion (DD) and AGD. Results : Overall 23/105 CPE (21.9%) were susceptible to mecillinam. Susceptibility was observed in E. coli ( N = 12), E. cloacae ( N = 7), and Klebsiella pneumoniae ( N = 4) producing IMI, OXA-48, OXA-48-like, and NDM-1 carbapenemases. MIC 50 for mecillinam in all isolates was 128 mg/L while MIC 50 for meropenem was 8 mg/L. Lower MICs for mecillinam were found in IMI (MIC 50 8 mg/L) and OXA-48-like (MIC 50 16 mg/L) producers. The comparison of the different susceptibility methods showed very major errors of 12.2% with AGD and 8.5% with disk diffusion when compared to the reference method. Conclusion : Mecillinam susceptibility was restricted to isolates producing IMI-, OXA-48-like, and NDM-1 carbapenemases and was documented despite high carbapenem MICs in some isolates. Mecillinam could be a promising oral antimicrobial in uUTI caused by E. coli and E. cloacae isolates carrying IMI- and OXA-48-like carbapenemases; however, susceptibility testing by AGD and disk diffusion remains problematic. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.3389/fmicb.2020.627267 |
| DOI: | 10.3389/fmicb.2020.627267/full |
| الاتاحة: | http://dx.doi.org/10.3389/fmicb.2020.627267 https://www.frontiersin.org/articles/10.3389/fmicb.2020.627267/full |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.703B7754 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fmicb.2020.627267 |
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