Academic Journal
STIM1/Orai1 contributes to sex differences in vascular responses to calcium in spontaneously hypertensive rats. Clin. Sci. (Lond
| العنوان: | STIM1/Orai1 contributes to sex differences in vascular responses to calcium in spontaneously hypertensive rats. Clin. Sci. (Lond |
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| المؤلفون: | Fernanda R C Giachini, Victor V Lima, Fernando P Filgueira, Anne M Dorrance, Maria Helena, C Carvalho, Zuleica B Fortes, R Clinton, Webb, Rita C Tostes |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.clinsci.org/content/ppclinsci/122/5/215.full.pdf. |
| سنة النشر: | 2012 |
| المجموعة: | CiteSeerX |
| الوصف: | Sex differences in Ca 2 + -dependent signalling and homoeostasis in the vasculature of hypertensive rats are well characterized. However, sex-related differences in SOCE (store-operated Ca 2 + entry) have been minimally investigated. We hypothesized that vascular protection in females, compared with males, reflects decreased Ca 2 + mobilization due to diminished activation of Orai1/STIM1 (stromal interaction molecule 1). In addition, we investigated whether ovariectomy in females affects the activation of the Orai1/STIM1 pathway. Endothelium-denuded aortic rings from male and female SHRSP (stroke-prone spontaneously hypertensive rats) and WKY (Wistar-Kyoto) rats and from OVX (ovariectomized) or sham female SHRSP and WKY rats were used to functionally evaluate Ca 2 + influx-induced contractions. Compared with females, aorta from male SHRSP displayed: (i) increased contraction during the Ca 2 + -loading period; (ii) similar transient contraction during Ca 2 + release from the intracellular stores; (iii) increased activation of STIM1 and Orai1, as shown by the blockade of STIM1 and Orai1 with neutralizing antibodies, which reversed the sex differences in contraction during the Ca 2 + -loading period; and (iv) increased expression of STIM1 and Orai1. Additionally, we found that aortas from OVX-SHRSP showed increased contraction during the Ca 2 + -loading period and increased Orai1 expression, but no changes in the SR (sarcoplasmic reticulum)-buffering capacity or STIM1 expression. These findings suggest that augmented activation of STIM1/Orai1 in aortas from male SHRSP represents a mechanism that contributes to sex-related impaired control of intracellular Ca 2 + levels. Furthermore, female sex hormones may negatively modulate the STIM/Orai1 pathway, contributing to vascular protection observed in female rats. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1076.8716; http://www.clinsci.org/content/ppclinsci/122/5/215.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.1076.8716 http://www.clinsci.org/content/ppclinsci/122/5/215.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.6F17F542 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |