التفاصيل البيبلوغرافية
| العنوان: |
Synthetic extracellular matrices and astrocytes provide a supportive microenvironment for the cultivation and investigation of primary pediatric gliomas |
| المؤلفون: |
Rota, Christopher M, Brown, Alexander T, Addleson, Emily, Ives, Clara, Trumper, Ella, Pelton, Kristine, Teh, Wei Pin, Schniederjan, Matthew J, Castellino, Robert Craig, Buhrlage, Sara, Lauffenburger, Douglas A, Ligon, Keith L, Griffith, Linda G, Segal, Rosalind A |
| المساهمون: |
Massachusetts Institute of Technology. Department of Biological Engineering |
| المصدر: |
Oxford University Press |
| بيانات النشر: |
Oxford University Press (OUP) |
| سنة النشر: |
2023 |
| المجموعة: |
DSpace@MIT (Massachusetts Institute of Technology) |
| الوصف: |
Background. Pediatric gliomas comprise a diverse set of brain tumor entities that have substantial long-term ramifications for patient survival and quality of life. However, the study of these tumors is currently limited due to a lack of authentic models. Additionally, many aspects of pediatric brain tumor biology, such as tumor cell invasiveness, have been difficult to study with currently available tools. To address these issues, we developed a synthetic extracellular matrix (sECM)-based culture system to grow and study primary pediatric brain tumor cells. Methods. We developed a brain-like sECM material as a supportive scaffold for the culture of primary, patientderived pediatric glioma cells and established patient-derived cell lines. Primary juvenile brainstem-derived murine astrocytes were used as a feeder layer to support the growth of primary human tumor cells. Results. We found that our culture system facilitated the proliferation of various primary pediatric brain tumors, including low-grade gliomas, and enabled ex vivo testing of investigational therapeutics. Additionally, we found that tuning this sECM material allowed us to assess high-grade pediatric glioma cell invasion and evaluate therapeutic interventions targeting invasive behavior. Conclusion. Our sECM culture platform provides a multipurpose tool for pediatric brain tumor researchers that enables both a wide breadth of biological assays and the cultivation of diverse tumor types. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Neuro-Oncology Advances; https://hdl.handle.net/1721.1/147854; Rota, Christopher M, Brown, Alexander T, Addleson, Emily, Ives, Clara, Trumper, Ella et al. 2022. "Synthetic extracellular matrices and astrocytes provide a supportive microenvironment for the cultivation and investigation of primary pediatric gliomas." Neuro-Oncology Advances, 4 (1). |
| الاتاحة: |
https://hdl.handle.net/1721.1/147854 |
| Rights: |
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. |
| رقم الانضمام: |
edsbas.6EB46212 |
| قاعدة البيانات: |
BASE |