Academic Journal
Recessive mutations in a common pathway block thymocyte apoptosis induced by multiple signals.
| العنوان: | Recessive mutations in a common pathway block thymocyte apoptosis induced by multiple signals. |
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| المؤلفون: | Flomerfelt, F A, Miesfeld, R L |
| المصدر: | The Journal of cell biology ; volume 127, issue 6, page 1729-1742 ; ISSN 0021-9525 1540-8140 |
| بيانات النشر: | Rockefeller University Press |
| سنة النشر: | 1994 |
| الوصف: | The glucocorticoid receptor (GR) is a ligand-regulated transcription factor that controls genes necessary to initiate glucocorticoid-induced thymocyte apoptosis. We have performed a genetic analysis of thymocyte cell death by isolating and characterizing a panel of GR+ dexamethasone-resistant mutants of the murine WEHI7.2 thymocyte cell line. These apoptosis-defective (Apt-) mutants were used to identify previously unknown early steps in the apoptotic pathway. The Apt- mutants contain nonglucocorticoid receptor, recessive mutations in genes that represent multiple complementation groups. These mutations block apoptosis induced by dexamethasone, gamma irradiation, and c-AMP treatment before the point where Bcl-2 exerts its protective effect. We propose that different signals share a common apoptotic pathway, and that the induction of apoptosis involves multiple precommitment steps that can be blocked by recessive mutations. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1083/jcb.127.6.1729 |
| الاتاحة: | http://dx.doi.org/10.1083/jcb.127.6.1729 https://rupress.org/jcb/article-pdf/127/6/1729/1478290/1729.pdf |
| رقم الانضمام: | edsbas.6E62F46F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1083/jcb.127.6.1729 |
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