Academic Journal
Characteristic of the active substance of the Saccharomyces cerevisiae preparation having radioprotective properties ; Характеристика активной субстанции препарата дрожжей Saccharomyces сerevisiae, обладающей радиопротекторными свойствами
| العنوان: | Characteristic of the active substance of the Saccharomyces cerevisiae preparation having radioprotective properties ; Характеристика активной субстанции препарата дрожжей Saccharomyces сerevisiae, обладающей радиопротекторными свойствами |
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| المؤلفون: | G. S. Ritter, V. P. Nikolin, N. A. Popova, A. S. Proskurina, P. E. Kisaretova, O. S. Taranov, T. D. Dubatolova, E. V. Dolgova, E. A. Potter, S. S. Kirikovich, Y. R. Efremov, S. I. Bayborodin, M. V. Romanenko, M. I. Meschaninova, A. G. Venyaminova, N. A. Kolchanov, S. S. Bogachev, Г. С. Риттер, В. П. Николин, Н. А. Попова, А. С. Проскурина, П. Э. Кисаретова, О. С. Таранов, Т. Д. Дубатолова, Е. В. Долгова, Е. А. Поттер, С. С. Кирикович, Я. Р. Ефремов, С. И. Байбородин, М. В. Романенко, М. И. Мещанинова, А. Г. Веньяминова, Н. А. Колчанов, С. С. Богачев |
| المساهمون: | This work was supported by State Budgeted Project No. 0324-2019-0042-C-01 for the Institute of Cytology and Genetics, Novosibirsk, registration ID AAAA-А17-117071240065-4, and the Russian Foundation for Basic Research, project No. 18-34-00205. Microscopical examination of cells was supported by State Budgeted Project No. 0310-2019-0005 for the Institute of Molecular and Cellular Biology, Novosibirsk. |
| المصدر: | Vavilov Journal of Genetics and Breeding; Том 24, № 6 (2020); 643-652 ; Вавиловский журнал генетики и селекции; Том 24, № 6 (2020); 643-652 ; 2500-3259 ; 2500-0462 ; 10.18699/VJ20.647 |
| بيانات النشر: | Institute of Cytology and Genetics of Siberian Branch of the RAS |
| سنة النشر: | 2020 |
| المجموعة: | Vavilov Journal of Genetics and Breeding / Вавиловский журнал генетики и селекции |
| مصطلحات موضوعية: | двуцепочечные разрывы, B-190, spleen colonies, double-stranded breaks, Б-190, селезеночные колонии |
| الوصف: | The paper describes some biological features of the radioprotective effect of double-stranded RNA preparation. It was found that yeast RNA preparation has a prolonged radioprotective effect after irradiation by a lethal dose of 9.4 Gy. 100 % of animals survive on the 70th day of observation when irradiated 1 hour or 4 days after 7 mg RNA preparation injection, 60 % animals survive when irradiated on day 8 or 12. Time parameters of repair of double-stranded breaks induced by gamma rays were estimated. It was found that the injection of the RNA preparation at the time of maximum number of double-stranded breaks, 1 hour after irradiation, reduces the efficacy of radioprotective action compared with the injection 1 hour before irradiation and 4 hours after irradiation. A comparison of the radioprotective effect of the standard radioprotector B-190 and the RNA preparation was made in one experiment. It has been established that the total RNA preparation is more efficacious than B-190. Survival on the 40th day after irradiation was 78 % for the group of mice treated with the RNA preparation and 67 % for those treated with B-190. In the course of analytical studies of the total yeast RNA preparation, it was found that the preparation is a mixture of single-stranded and double-stranded RNA. It was shown that only double-stranded RNA has radioprotective properties. Injection of 160 μg double-stranded RNA protects 100 % of the experimental animals from an absolutely lethal dose of gamma radiation, 9.4 Gy. It was established that the radioprotective effect of double-stranded RNA does not depend on sequence, but depends on its double-stranded form and the presence of “open” ends of the molecule. It is supposed that the radioprotective effect of double-stranded RNA is associated with the participation of RNA molecules in the correct repair of radiation-damaged chromatin in blood stem cells. The hematopoietic pluripotent cells that have survived migrate to the periphery, reach the spleen and actively proliferate. The newly ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Russian |
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| DOI: | 10.18699/VJ20.658 |
| الاتاحة: | https://vavilov.elpub.ru/jour/article/view/2780 https://doi.org/10.18699/VJ20.658 |
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| رقم الانضمام: | edsbas.6E50498D |
| قاعدة البيانات: | BASE |
| DOI: | 10.18699/VJ20.658 |
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