Academic Journal

Additional file 1 of LEA motifs promote desiccation tolerance in vivo

التفاصيل البيبلوغرافية
العنوان: Additional file 1 of LEA motifs promote desiccation tolerance in vivo
المؤلفون: Jonathan D. Hibshman (11833785), Bob Goldstein (265221)
سنة النشر: 2021
المجموعة: Smithsonian Institution: Digital Repository
مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Molecular Biology, Immunology, Developmental Biology, Cancer, Mental Health, Infectious Diseases, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Late embryogenesis abundant (LEA), Desiccation, Osmotic stress, C. elegans, Stress
الوصف: Additional file 1: Figure S1. Genotyping LEA-1 alleles. Figure S2. Representative images of dauer-like larvae of lea-1 mutants. Figure S3. Mutations in lea-1 impact wild-type worms (N2) similarly to daf-2 mutants. Figure S4. LEA-1 does not significantly alter polyglutamine protein aggregation due to age. Figure S5. LEA-1 expression does not increase in response to short-term osmotic stress. Figure S6. Representative images of auxin-induced depletion of mNG::lea-1 in TIR1 expressing strains. Figure S7. Overexpression of lea-1a in body wall muscle does not rescue SDS sensitivity, desiccation tolerance, or osmotic stress resistance. Figure S8. Controls for heterologous expression of proteins in bacteria. Figure S9. Characterization of LEA-1 motif-expressing worms. Figure S10. Expression of LEA-1 motifs in body wall muscle does not improve SDS resistance, desiccation tolerance, or osmotic stress survival. Table S1. Genotyping primers for lea-1 genomic edits. Table S2. Lifespan analysis of lea-1 mutants.
نوع الوثيقة: article in journal/newspaper
اللغة: unknown
Relation: https://figshare.com/articles/journal_contribution/Additional_file_1_of_LEA_motifs_promote_desiccation_tolerance_in_vivo/17194170
DOI: 10.6084/m9.figshare.17194170.v1
الاتاحة: https://doi.org/10.6084/m9.figshare.17194170.v1
Rights: CC BY + CC0
رقم الانضمام: edsbas.6E1A66BB
قاعدة البيانات: BASE
الوصف
DOI:10.6084/m9.figshare.17194170.v1