التفاصيل البيبلوغرافية
| العنوان: |
Exploring Covalent Bond Formation at Tyr-82 for Inhibition of Ral GTPase Activation |
| المؤلفون: |
Landgraf, Alexander D., Yeh, I-Ju, Ghozayel, Mona K., Bum-Erdene, Khuchtumur, Gonzalez-Gutierrez, Giovanni, Meroueh, Samy O. |
| المساهمون: |
Biochemistry and Molecular Biology, School of Medicine |
| المصدر: |
PMC |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2023 |
| المجموعة: |
Indiana University - Purdue University Indianapolis: IUPUI Scholar Works |
| مصطلحات موضوعية: |
Pancreatic ductal carcinoma, GTP phosphohydrolases, Guanine nucleotide exchange factors, Pancreatic neoplasms |
| الوصف: |
Ral RAS GTPases are directly activated by KRAS through a trimeric complex with a guanine exchange factor. Ral is considered undruggable and lacks an accessible cysteine for covalent drug development. Previously we had reported an aryl sulfonyl fluoride fragment that formed a covalent bond at Tyr-82 on Ral and created a deep and well-defined pocket. Here, we explore this pocket further through design and synthesis of several fragment derivatives. The fragment core is modified by introducing tetrahydronaphthalene or benzodioxane rings to enhance affinity and stability of the sulfonyl fluoride reactive group. The deep pocket in the Switch II region is also explored by modifying the aromatic ring of the fragment that is ensconced into the pocket. Compounds 19 (SOF-658) and 26 (SOF-648) formed a single robust adduct specifically at Tyr-82, inhibited Ral GTPase exchange in buffer and in mammalian cells, and blocked invasion of pancreatic ductal adenocarcinoma cancer cells. Compound 19 (SOF-658) was stable in buffer, mouse, and human microsomes suggesting that further optimization could lead to small molecules to probe Ral activity in tumor models. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
ChemMedChem; Landgraf AD, Yeh IJ, Ghozayel MK, Bum-Erdene K, Gonzalez-Gutierrez G, Meroueh SO. Exploring Covalent Bond Formation at Tyr-82 for Inhibition of Ral GTPase Activation. ChemMedChem. 2023;18(16):e202300272. doi:10.1002/cmdc.202300272; https://hdl.handle.net/1805/41399 |
| الاتاحة: |
https://hdl.handle.net/1805/41399 |
| Rights: |
Attribution-NonCommercial-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.6DD09298 |
| قاعدة البيانات: |
BASE |