Academic Journal
Cellular Synthesis of Protein Catenanes
| العنوان: | Cellular Synthesis of Protein Catenanes |
|---|---|
| المؤلفون: | Wang, Xiao-Wei, Zhang, Wen-Bin |
| المساهمون: | Zhang, WB (reprint author), Peking Univ, Key Lab Polymer Chem & Phys, Ctr Soft Matter Sci & Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China., Peking Univ, Key Lab Polymer Chem & Phys, Ctr Soft Matter Sci & Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China. |
| المصدر: | PubMed ; SCI ; EI |
| بيانات النشر: | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
| سنة النشر: | 2016 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | catenanes, protein structures, protein folding, supramolecular chemistry, topology, SPYTAG-SPYCATCHER CHEMISTRY, CHEMICAL-SYNTHESIS, CIRCULAR PROTEINS, NATURAL-PRODUCTS, PEPTIDE TAG, KNOTS, CYCLIZATION, STABILITY, CHAINS, FORMS |
| الوصف: | Direct cellular production of topologically complex proteins is of great interest both in supramolecular chemistry and protein engineering. We describe the first cellular synthesis of protein catenanes through the use of the p53 dimerization domain to guide the intertwining of two protein chains and SpyTag-SpyCatcher chemistry for efficient cyclization. The catenane topology was unambiguously proven by SDS-PAGE, SEC, and partial digestion experiments and was shown to confer enhanced stability toward trypsin digestion relative to monomeric control mutants. The assembly-reaction synergy enabled by protein folding and genetically encoded protein chemistry offers a convenient yet powerful approach for creating mechanically interlocked, complex protein topologies invivo. ; 863 Program [2015AA020941]; National Natural Science Foundation of China [21474003, 91427304]; 1000 Plan (Youth) ; SCI(E) ; EI ; PubMed ; ARTICLE ; wenbin@pku.edu.cn ; 10 ; 3442-3446 ; 55 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | Chinese |
| تدمد: | 1433-7851 1521-3773 26833700 |
| Relation: | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.2016,55,(10),3442-3446.; 1391026; http://hdl.handle.net/20.500.11897/435116; WOS:000371418200040 |
| DOI: | 10.1002/anie.201511640 |
| الاتاحة: | https://hdl.handle.net/20.500.11897/435116 https://doi.org/10.1002/anie.201511640 |
| رقم الانضمام: | edsbas.6DB5344A |
| قاعدة البيانات: | BASE |
| تدمد: | 14337851 15213773 26833700 |
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| DOI: | 10.1002/anie.201511640 |