Academic Journal
A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
| العنوان: | A Robust Mammary Organoid System to Model Lactation and Involution-like Processes |
|---|---|
| المؤلفون: | Charifou, Elsa, Sumbal, Jakub, Koledova, Zuzana, Li, Han, Chiche, Aurélie |
| المساهمون: | Plasticité cellulaire et Modélisation des Maladies / Cellular Plasticity and Disease Modelling, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Masaryk University Brno (MUNI), Work in the laboratory of HL is funded by the Pasteur, Centre National pour la Recherche the Agence Nationale de la Recherche (ANR-10-LABX-73 and ANR-16-CE13-0017- 01), Fondation ARC (PJA 20161205028 and 20181208231), Programme Barrande, a nd AFM-Telethon Foundation. AC was funded by postdoctoral fellowships from the Revive Consortium. EC was funded by a Ph.D. fellowship from Sorbonne Université. ZK was funded by the Grant Agency of Masaryk University (MUNI/G/1446/2018), Mobility grant by Ministry of Education, and Youth and Sports, and by funds from the Faculty of Medicine MU to the junior researcher (ROZV/28/LF/2020). JS was funded by the P- Pool (Faculty of Medicine MU) and the Grant Agency of Masaryk University (MUNI/A/1565/2018, ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), ANR-16-CE13-0017,iCELLPLASTICITY,Plasticité cellulaire dans le vieillissement(2016) |
| المصدر: | ISSN: 2331-8325 ; Bio-protocol ; https://hal-pasteur.archives-ouvertes.fr/pasteur-03287059 ; Bio-protocol , 2021, 11 (8), pp.e3996. ⟨10.21769/BioProtoc.3996⟩. |
| بيانات النشر: | HAL CCSD Bio-protocol LCC |
| سنة النشر: | 2021 |
| المجموعة: | Institut Pasteur: HAL |
| مصطلحات موضوعية: | Mouse, Mammary gland, 3D organoid, Ex vivo, Lactation, Involution, [SDV]Life Sciences [q-bio], [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], [SDV.IB]Life Sciences [q-bio]/Bioengineering, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials |
| الوصف: | International audience ; The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding how dysregulation can lead to breast cancer initiation and progression. Three-dimensional (3D) mammary organoids offer many exciting possibilities for the study of tissue development and breast cancer. In the present protocol derived from Sumbal et al., we describe a straightforward 3D organoid system for the study of lactation and involution ex vivo. We use primary and passaged mouse mammary organoids stimulated with fibroblast growth factor 2 (FGF2) and prolactin to model the three cycles of mouse mammary gland lactation and involution processes. This 3D organoid model represents a valuable tool to study late postnatal mammary gland development and breast cancer, in particular postpartum-associated breast cancer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34124297; pasteur-03287059; https://hal-pasteur.archives-ouvertes.fr/pasteur-03287059; https://hal-pasteur.archives-ouvertes.fr/pasteur-03287059/document; https://hal-pasteur.archives-ouvertes.fr/pasteur-03287059/file/2021%20-%20Charifou%20et%20al.,%20Bio-protocol.pdf; PUBMED: 34124297; PUBMEDCENTRAL: PMC8160540 |
| DOI: | 10.21769/BioProtoc.3996 |
| الاتاحة: | https://hal-pasteur.archives-ouvertes.fr/pasteur-03287059 https://hal-pasteur.archives-ouvertes.fr/pasteur-03287059/document https://hal-pasteur.archives-ouvertes.fr/pasteur-03287059/file/2021%20-%20Charifou%20et%20al.,%20Bio-protocol.pdf https://doi.org/10.21769/BioProtoc.3996 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6D60A732 |
| قاعدة البيانات: | BASE |
| DOI: | 10.21769/BioProtoc.3996 |
|---|