التفاصيل البيبلوغرافية
| العنوان: |
Quality Assurance and Cost Reduction in Histopathology Laboratories Using Tissue Microarrays |
| المؤلفون: |
Daniela Gologan, Alina Elena Ștefan, Manuella Militaru, Andreea Cristina Sanda, Suzana Arjan, Sorin Mușat, Matthew Okerlund Leavitt, Raluca Stan |
| المصدر: |
Veterinary Sciences; Volume 10; Issue 4; Pages: 280 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
tissue microarray, quality assurance, cost-reduction, tissue processing protocol, multiplexing |
| جغرافية الموضوع: |
agris |
| الوصف: |
In the context of cost increases of both labor and consumables, cheaper and faster histopathology methods are needed. We implemented in our research laboratory the use of tissue microarrays (TMA) for the parallel processing and analysis of tissue samples. In this study, we used seven pre-processed, paraffinated biomimetic sectionable support matrices serving as “recipient” paraffin blocks to embed a total of 196 tissue cores from formalin-fixed paraffin-embedded tissue samples (serving as “donor” paraffin blocks) from seven different rabbit organs. These tissue samples were obtained using four different processing protocols: two 6 h protocols with xylene as the transition solvent, and two using butanol instead (one 10 h in duration and the other 72 h long). While the samples from protocols 1 and 2 (with xylene) quite regularly generated peeling of some of the cores from the slides (most likely because of substandard paraffin infiltration), butanol processing performed flawlessly for both processing protocols. Our proposed technique of using TMAs in the research laboratory brings with it a significant reduction in time and consumable costs (up to 77 and 64%, respectively), but also new challenges for all the upstream processes. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Anatomy, Histology and Pathology; https://dx.doi.org/10.3390/vetsci10040280 |
| DOI: |
10.3390/vetsci10040280 |
| الاتاحة: |
https://doi.org/10.3390/vetsci10040280 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.6D387ED4 |
| قاعدة البيانات: |
BASE |