Academic Journal
Interplay between mistranslation and oxidative stress in Escherichia coli ; Međuigra mistranslacije i oksidacijskog stresa u bakteriji Escherichia coli
| العنوان: | Interplay between mistranslation and oxidative stress in Escherichia coli ; Međuigra mistranslacije i oksidacijskog stresa u bakteriji Escherichia coli |
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| المؤلفون: | Ević, Valentina, Rokov-Plavec, Jasmina |
| المصدر: | Arhiv za higijenu rada i toksikologiju ; ISSN 0004-1254 (Print) ; ISSN 1848-6312 (Online) ; Volume 75 ; Issue 2 |
| بيانات النشر: | Institute for Medical Research and Occupational Health |
| سنة النشر: | 2024 |
| المجموعة: | Hrčak - Portal of scientific journals of Croatia / Portal znanstvenih časopisa Republike Hrvatske |
| مصطلحات موضوعية: | aminoacyl-tRNA synthetase, hydrogen peroxide, isoleucyl-tRNA synthetase, stress response, translation, aminoacil-tRNA-sintetaza, izoleucil-tRNA-sintetaza, odgovor na stres, translacija, vodikov peroksid |
| الوصف: | Mistakes in translation are mostly associated with toxic effects in the cell due to the production of functionally aberrant and misfolded proteins. However, under certain circumstances mistranslation can have beneficial effects and enable cells to preadapt to other stress conditions. Mistranslation may be caused by mistakes made by aminoacyl-tRNA synthetases, essential enzymes that link amino acids to cognate tRNAs. There is an Escherichia coli strain expressing isoleucyl-tRNA synthetase mutant variant with inactivated editing domain which produces mistranslated proteomes where valine (Val) and norvaline (Nva) are misincorporated into proteins instead of isoleucine. We compared this strain with the wild-type to determine the effects of such mistranslation on bacterial growth in oxidative stress conditions. When the cells were pre-incubated with 0.75 mmol/L Nva or 1.5 mmol/L Val or Nva and exposed to hydrogen peroxide, no beneficial effect of mistranslation was observed. However, when the editing-deficient strain was cultivated in medium supplemented with 0.75 mmol/L Val up to the early or mid-exponential phase of growth and then exposed to oxidative stress, it slightly outgrew the wild-type grown in the same conditions. Our results therefore show a modest adaptive effect of isoleucine mistranslation on bacterial growth in oxidative stress, but only in specific conditions. This points to a delicate balance between deleterious and beneficial effects of mistranslation. ; Pogreške u translaciji uglavnom su povezane s toksičnim učincima u stanici zbog proizvodnje funkcionalno aberantnih i pogrešno smotanih proteina. Međutim, pod određenim okolnostima pogreške u translaciji mogu imati povoljne učinke i omogućiti stanicama da se prilagode drugim stresnim uvjetima. Mistranslacija može biti prouzročena pogreškama koje čine aminoacil-tRNA-sintetaze, esencijalni enzimi koji povezuju aminokiseline s pripadnim molekulama tRNA. Soj bakterije Escherichia coli koji eksprimira mutiranu inačicu izoleucil-tRNAsintetaze s ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://hrcak.srce.hr/318103 |
| الاتاحة: | https://hrcak.srce.hr/318103 https://hrcak.srce.hr/file/459717 |
| Rights: | info:eu-repo/semantics/openAccess ; Copyright: Institute for Medical Research and Occupational Health, Zagreb, Croatia Parts of the contents such as figures and tables may be reproduced without prior permission, provided reference is made to the source. Annual subscription to the printed version (postage included): Europe Eur 70.00; overseas countries Eur 85,00 Slovenian Society of Toxicology Eur 30.00 Full text articles online available for free. |
| رقم الانضمام: | edsbas.6D2892BA |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |