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MoMkk1-dependent MoAtg4 phosphorylation negatively regulates autophagy under cell wall stress.
العنوان: | MoMkk1-dependent MoAtg4 phosphorylation negatively regulates autophagy under cell wall stress. |
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المؤلفون: | Pusheng Guo, Yurong Wang, Jiayun Xu, Zhixiang Yang, Ziqi Zhang, Jinyi Qian, Jiexiong Hu, Ziyi Yin, Leiyun Yang, Muxing Liu, Xinyu Liu, Gang Li, Haifeng Zhang, Ryan Rumsey, Ping Wang, Zhengguang Zhang |
سنة النشر: | 2024 |
مصطلحات موضوعية: | Biochemistry, Medicine, Microbiology, Cell Biology, Physiology, Immunology, Developmental Biology, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, rice blast fungus, regulation remains unknown, div >< p, cell wall integrity, also phosphorylates moatg4, atg protein moatg4, magnaporthe oryzae <, oryzae <, moatg4 phosphorylation, terminal cleavage, responsive processes, regulate virulence, important process, host plants, fungal infection, critical stress, core kinase |
الوصف: | (A) AB observation after MDC staining under CFW treatment. C: cytoplasm. V: vacuole. (B) Quantification of vacuoles with AB as shown in (A). Different letters indicate statistically significant differences (Duncan’s new multiple range test, p < 0.01). (C) An amino acid sequence alignment of M . oryzae MoAtg8 and S . cerevisiae Atg8. (D) Cleavage assay of MoAtg8-RFP in mycelia following treatment with CFW. (E) MoAtg8-RFP localization was observed at the appressorium stage. (F) Cleavage assay of MoAtg8-RFP at the conidia stage. (G) Co-IP assay to analyze the interaction between MoAtg4 3A or MoAtg4 3D , and MoAtg8 under CFW treatment. (H) Interaction analysis of MoAtg4 3A and MoAtg4 3D with MoMkk1 by Y2H. CFW treatment: treated with 1 mg/ml CFW for 5 h. Scale bar: 5 μm. (TIF) |
نوع الوثيقة: | still image |
اللغة: | unknown |
Relation: | https://figshare.com/articles/figure/MoMkk1-dependent_MoAtg4_phosphorylation_negatively_regulates_autophagy_under_cell_wall_stress_/25114063 |
DOI: | 10.1371/journal.ppat.1011988.s005 |
الاتاحة: | https://doi.org/10.1371/journal.ppat.1011988.s005 https://figshare.com/articles/figure/MoMkk1-dependent_MoAtg4_phosphorylation_negatively_regulates_autophagy_under_cell_wall_stress_/25114063 |
Rights: | CC BY 4.0 |
رقم الانضمام: | edsbas.6CF0153E |
قاعدة البيانات: | BASE |
DOI: | 10.1371/journal.ppat.1011988.s005 |
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