Academic Journal
Host-pathogen interaction: Enterobacter cloacae exerts different adhesion and invasion capacities against different host cell types
| العنوان: | Host-pathogen interaction: Enterobacter cloacae exerts different adhesion and invasion capacities against different host cell types |
|---|---|
| المؤلفون: | Frutos-Grilo, Elisabet, Kreling, Vanessa, Hensel, Andreas, Campoy, Susana |
| المساهمون: | Asare, Kwame Kumi, Fundació la Marató de TV3, EMBO |
| المصدر: | PLOS ONE ; volume 18, issue 10, page e0289334 ; ISSN 1932-6203 |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2023 |
| المجموعة: | PLOS Publications (via CrossRef) |
| الوصف: | New antibiotics are urgently needed due to the huge increase of multidrug-resistant bacteria. The underexplored gram-negative bacterium Enterobacter cloacae is known to cause severe urinary tract and lung infections (UTIs). The pathogenicity of E . cloacae in UTI has only been studied at the bioinformatic level, but until now not within systematic in vitro investigations. The present study assesses different human cell lines for monitoring the early steps of host-pathogen interaction regarding bacterial adhesion to and invasion into different host cells by flow cytometric adhesion assay, classical cell counting assay, gentamicin invasion assay, and confocal laser scanning microscopy. To our knowledge, this is the first report in which E . cloacae has been investigated for its interaction with human bladder, kidney, skin, and lung cell lines under in vitro conditions. Data indicate that E . cloacae exerts strong adhesion to urinary tract (bladder and kidney) and lung cells, a finding which correlates with the clinical relevance of the bacterium for induction of urinary tract and lung infections. Furthermore, E . cloacae ATCC 13047 barely adheres to skin cells (A-431) and shows no relevant interaction with intestinal cells (Caco-2, HT-29), even in the presence of mucin (HT29 MTX). In contrast, invasion assays and confocal laser scanning microscopy demonstrate that E . cloacae internalizes in all tested host cells, but to a different extent. Especially, bladder and kidney cells are being invaded to the highest extent. Defective mutants of fimH and fimA abolished the adhesion of E . cloacae to T24 cells, while csgA deletion had no influence on adhesion. These results indicate that E . cloacae has different pattern for adhesion and invasion depending on the target tissue, which again correlates with the clinical relevance of the pathogen. For detailed investigation of the early host-pathogen interaction T24 bladder cells comprise a suitable assay system for evaluation the bacterial adhesion and invasion. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1371/journal.pone.0289334 |
| الاتاحة: | http://dx.doi.org/10.1371/journal.pone.0289334 https://dx.plos.org/10.1371/journal.pone.0289334 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.6CAE9BE7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0289334 |
|---|