Academic Journal
Catalase Detection via Membrane-Based Pressure Sensors
| العنوان: | Catalase Detection via Membrane-Based Pressure Sensors |
|---|---|
| المؤلفون: | Monica Bianco, Alessandra Zizzari, Elisabetta Perrone, Diego Mangiullo, Marco Mazzeo, Ilenia Viola, Valentina Arima |
| المصدر: | Molecules, Vol 29, Iss 7, p 1506 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | pressure sensors, PDMS membranes, catalase, bioassays, membrane deflection, Organic chemistry, QD241-441 |
| الوصف: | Membrane-based sensors (MePSs) exhibit remarkable precision and sensitivity in detecting pressure changes. MePSs are commonly used to monitor catalytic reactions in solution, generating gas products crucial for signal amplification in bioassays. They also allow for catalyst quantification by indirectly measuring the pressure generated by the gaseous products. This is particularly interesting for detecting enzymes in biofluids associated with disease onset. To enhance the performance of a MePS, various structural factors influence membrane flexibility and response time, ultimately dictating the device’s pressure sensitivity. In this study, we fabricated MePSs using polydimethylsiloxane (PDMS) and investigated how structural modifications affect the Young’s modulus ( E ) and residual stress ( σ 0 ) of the membranes. These modifications have a direct impact on the sensors’ sensitivity to pressure variations, observed as a function of the volume of the chamber ( Σ ) or of the mechanical properties of the membrane itself ( S ). MePSs exhibiting the highest sensitivities were then employed to detect catalyst quantities inducing the dismutation of hydrogen peroxide, producing dioxygen as a gaseous product. As a result, a catalase enzyme was successfully detected using these optimized MePSs, achieving a remarkable sensitivity of (22.7 ± 1.2) µm/nM and a limit of detection (LoD) of 396 pM. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1420-3049 |
| Relation: | https://www.mdpi.com/1420-3049/29/7/1506; https://doaj.org/toc/1420-3049; https://doaj.org/article/6c3bdae22dea41eeb3bbf8c0c1c4886b |
| DOI: | 10.3390/molecules29071506 |
| الاتاحة: | https://doi.org/10.3390/molecules29071506 https://doaj.org/article/6c3bdae22dea41eeb3bbf8c0c1c4886b |
| رقم الانضمام: | edsbas.6C333CE1 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14203049 |
|---|---|
| DOI: | 10.3390/molecules29071506 |