Academic Journal
1α,25‐Dihydroxy‐3‐epi‐vitamin D 3 : In vivo metabolite of 1α,25‐dihydroxyvitamin D 3 in rats
| العنوان: | 1α,25‐Dihydroxy‐3‐epi‐vitamin D 3 : In vivo metabolite of 1α,25‐dihydroxyvitamin D 3 in rats |
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| المؤلفون: | Sekimoto, H, Siu-Caldera, M.-L, Weiskopf, A, Vouros, P, Muralidharan, K.R, Okamura, W.H, Uskokovic, M.R, Reddy, G.S |
| المصدر: | FEBS Letters ; volume 448, issue 2-3, page 278-282 ; ISSN 0014-5793 1873-3468 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1999 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | We recently identified 1α,25‐dihydroxy‐3‐epi‐vitamin D 3 as a major in vitro metabolite of 1α,25‐dihydroxyvitamin D 3 , produced in primary cultures of neonatal human keratinocytes. We now report the isolation of 1α,25‐dihydroxy‐3‐epi‐vitamin D 3 from the serum of rats treated with pharmacological doses of 1α,25‐dihydroxyvitamin D 3 . 1α,25‐dihydroxy‐3‐epi‐vitamin D 3 was identified through its co‐migration with synthetic 1α,25‐dihydroxy‐3‐epi‐vitamin D 3 on both straight and reverse phase high performance liquid chromatography systems and by mass spectrometry. Along with 1α,25‐dihydroxy‐3‐epi‐vitamin D 3 , other previously known metabolites, namely, 1α,24( R ),25‐trihydroxyvitamin D 3 , 1α,25‐dihydroxy‐24‐oxo‐vitamin D 3 and 1α,25‐dihydroxyvitamin D 3 ‐26,23‐lactone, were also identified. Thus, our study for the first time provides direct evidence to indicate that 1α,25‐dihydroxy‐3‐epi‐vitamin D 3 is an in vivo metabolite of 1α,25‐dihydroxyvitamin D 3 in rats. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1016/s0014-5793(99)00377-4 |
| DOI: | 10.1016/S0014-5793%2899%2900377-4 |
| الاتاحة: | http://dx.doi.org/10.1016/s0014-5793(99)00377-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793%2899%2900377-4 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(99)00377-4 https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/S0014-5793%2899%2900377-4 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.6C122218 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/s0014-5793(99)00377-4 |
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