Book
2D-NMR demonstration of lignin removal from wood and non-wood plant feedstocks by fungal versatile peroxidase
| العنوان: | 2D-NMR demonstration of lignin removal from wood and non-wood plant feedstocks by fungal versatile peroxidase |
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| المؤلفون: | Fernández-Fueyo, Elena, Rencoret, Jorge, Berbís, Manuel Álvaro, Ruiz-Dueñas, F. J., Gutiérrez Suárez, Ana, Río Andrade, José Carlos del, Jiménez-Barbero, Jesús, Hammel, Kenneth E., Martínez, Ángel T. |
| بيانات النشر: | CSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS) |
| سنة النشر: | 2014 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Lignin, Enzymes, Versatile peroxidase, Veratryl alcohol, 2D NMR, Eucalyptus |
| الوصف: | The ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escherichia coli and "in vitro" folded. Two-dimensional nuclear magnetic resonance (NMR) of the treated materials, swollen in dimethylsulfoxide-d6, revealed a reduction in the number of lignin side-chain linkages after both treatments. However, a significant removal of lignin by the enzymatic treatment was only observed in the case of eucalypt wood, as shown by the decrease of lignin aromatic signals referred to carbohydrate anomeric signals. ; E.F.-F. and J.R. thank Junta de Ampliación de Estudios of CSIC for a JAE PhD fellowship and a JAE-DOC contract (co-funded by the European Social Fund). M.A.B. acknowledges an FPI fellowship from the Spanish Ministry of Economy and Competitiveness. This work was supported by the Spanish HIPOP (BIO2011-26694) and LIGNOCELL (AGL2011-25379) projects (co-financed by FEDER funds) and the European projects PEROXICATS (KBBE-2010-4-265397) and INDOX (KBBE-2013-7-613549). ; Peer reviewed |
| نوع الوثيقة: | book part |
| اللغة: | English |
| ردمك: | 978-84-616-9842-4 84-616-9842-8 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; (KBBE-2013-7-613549; Sí; Proceedings of the 13th European Workshop on Lignocellulosics and Pulp, pp. 347-350 (2014); http://hdl.handle.net/10261/98704 |
| الاتاحة: | http://hdl.handle.net/10261/98704 |
| Rights: | open |
| رقم الانضمام: | edsbas.6BA4CD73 |
| قاعدة البيانات: | BASE |
| ردمك: | 9788461698424 8461698428 |
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