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Cell polarity defines three distinct domains in pancreatic beta cells

التفاصيل البيبلوغرافية
العنوان: Cell polarity defines three distinct domains in pancreatic beta cells
المؤلفون: Gan, Wan J., Zavortink, Michael, Ludick, Christine, Templin, Rachel, Webb, Robyn, Webb, Richard, Ma, Wei, Poronnik, Philip, Parton, Robert G., Gaisano, Herbert Y., Shewan, Annette M., Thorn, Peter
بيانات النشر: The Company of Biologists
سنة النشر: 2016
المجموعة: The University of Queensland: UQ eSpace
مصطلحات موضوعية: Insulin, Polarity, Islet, Diabetes, β-Cell, 1307 Cell Biology
الوصف: The structural organisation of pancreatic β-cells in the islets of Langerhans is relatively unknown. Here, using three-dimensional (3D) two-photon, 3D confocal and 3D block-face serial electron microscopy, we demonstrate a consistent in situ polarisation of β-cells and define three distinct cell surface domains. An apical domain located at the vascular apogee of β-cells, defined by the location of PAR-3 (also known as PARD3) and ZO-1 (also known as TJP1), delineates an extracellular space into which adjacent β-cells project their primary cilia. A separate lateral domain, is enriched in scribble and Dlg, and colocalises with E-cadherin and GLUT2 (also known as SLC2A2). Finally, a distinct basal domain, where the β-cells contact the islet vasculature, is enriched in synaptic scaffold proteins such as liprin. This 3D analysis of β-cells within intact islets, and the definition of distinct domains, provides new insights into understanding β-cell structure and function.
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 0021-9533
1477-9137
Relation: orcid:0000-0001-8638-1438; orcid:0000-0002-7494-5248; orcid:0000-0003-4936-9207; orcid:0000-0002-3228-770X; DP110100642; APP1002520; APP1059426; APP1037320; Y15G-THOP
الاتاحة: https://espace.library.uq.edu.au/view/UQ:381769
رقم الانضمام: edsbas.6B7278AD
قاعدة البيانات: BASE
الوصف
تدمد:00219533
14779137