Academic Journal
The role of the serum exosomal and endometrial microRNAs in recurrent implantation failure
| العنوان: | The role of the serum exosomal and endometrial microRNAs in recurrent implantation failure |
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| المؤلفون: | Baştu, Ercan, Günel, Tuba, Azhari, Fatemeh, Pence, Sadrettin, Hosseini, Mohammad Kazem, Balci, Burcin Karamustafaoglu, Cevik, Nazife |
| المساهمون: | Istanbul Üniversitesi Tıp Fakültesi ,, 2503545 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Pathophysiology, Pediatrics, Perinatology and Child Health, Internal Medicine, Assessment and Diagnosis, Medicine (miscellaneous), General Medicine, Health Sciences, Klinik Tıp (MED), Klinik Tıp, CERRAHİ, TIP, GENEL & İÇECEK, KADIN HASTALIKLARI & DOĞUM, PEDİATRİ, Tıp, Sağlık Bilimleri, Dahili Tıp Bilimleri, Çocuk Sağlığı ve Hastalıkları, Cerrahi Tıp Bilimleri, Kadın Hastalıkları ve Doğum, Family Practice, Obstetrics and Gynecology, Fundamentals and Skills, Surgery, General Health Professions |
| الوصف: | © 2020 Informa UK Limited, trading as Taylor & Francis Group.Objective: It has been identified that endometrium specific microRNAs have different expression levels in endometrial tissues and maternal serum during endometrial cycle. The aim of this study was to analyze microRNA expression levels in recurrent implantation failure patients and healthy controls endometrial samples for enlightening the aetiopathogenesis of the disease. The second aim was to search for a potential noninvasive molecular biomarker in early diagnosis and treatment of Recurrent Implantation Failure (RIF) patients. Methods: Endometrium and serum samples in two different phases (PP; proliferative phase and SP; secretory phase) from the same cases (RIF; n = 12 and Control; n = 8) were obtained. The expression levels of the microRNA by RT-qPCR method were measured. The expression levels of the healthy controls and study group were compared. Lastly performed target genes analysis of significantly dysregulated miRNA by target analyze databases for obtained related biological pathways. Results: This study showed that has-miR-145, has-miR-23b, has-miR-31 and has-miR-30b were significantly up-regulated in PP and down-regulated in SP endometrium samples. In serum samples, has-miR-145 and hsa-miR-23b were significantly down-regulated in both of PP and SP. Target gene and pathway analysis for dysregulated miRNAs identified important, validated and predicted genes for the implantation process. Conclusions: This study is the first study to obtain endometrium and serum samples in two different phases from the same cases and measure the candidate miRNAs expression. Our finding suggests that expression level of four candidate miRNAs may be involved in RIF development in women. Furthermore, these miRNAs can be potential biomarker for early diagnosis of RIF patients. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1476-7058 |
| Relation: | Journal of Maternal-Fetal and Neonatal Medicine; Azhari F., Pence S., Hosseini M. K. , Balci B. K. , Cevik N., Baştu E., Günel T., "The role of the serum exosomal and endometrial microRNAs in recurrent implantation failure", Journal of Maternal-Fetal and Neonatal Medicine, 2020; av_f25b5e91-7287-4575-9425-42b5c04bee88; vv_1032021; http://hdl.handle.net/20.500.12627/158968; https://doi.org/10.1080/14767058.2020.1849095 |
| DOI: | 10.1080/14767058.2020.1849095 |
| الاتاحة: | https://hdl.handle.net/20.500.12627/158968 https://doi.org/10.1080/14767058.2020.1849095 |
| رقم الانضمام: | edsbas.6B41926 |
| قاعدة البيانات: | BASE |
| تدمد: | 14767058 |
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| DOI: | 10.1080/14767058.2020.1849095 |