Academic Journal
Effect of ethanol perturbation on viscosity and permeability of an inner membrane in Bacillus subtilis spores.
| العنوان: | Effect of ethanol perturbation on viscosity and permeability of an inner membrane in Bacillus subtilis spores. |
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| المؤلفون: | Loison, P, Gervais, P, Perrier-Cornet, JM, Kuimova, MK |
| المساهمون: | Engineering & Physical Science Research Council (EPSRC) |
| المصدر: | 2069 ; 2060 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2016 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Bacillus subtilis spores, Ethanol perturbation, Fluorescence lifetime imaging (FLIM), Inner membrane, Microviscosity decrease, Permeabilization, 06 Biological Sciences, 02 Physical Sciences |
| الوصف: | In this work, we investigated how a combination of ethanol and high temperature (70°C), affect the properties of the inner membrane of Bacillus subtilis spores. We observed membrane permeabilization for ethanol concentrations ≥50%, as indicated by the staining of the spores DNA by the cell impermeable dye Propidium Iodide. The loss of membrane integrity was also confirmed by a decrease in the peak corresponding to dipicolinic acid using infrared spectroscopy. Finally, the spore refractivity (as measured by phase contrast microscopy) was decreased after the ethanol-heat treatment, suggesting a partial rehydration of the protoplast. Previously we have used fluorescent lifetime imaging microscopy (FLIM) combined with the fluorescent molecular rotor Bodipy-C12 to study the microscopic viscosity in the inner membrane of B. subtilis spores, and showed that at normal conditions it is characterized by a very high viscosity. Here we demonstrate that the ethanol/high temperature treatment led to a decrease of the viscosity of the inner membrane, from 1000cP to 860cP for wild type spores at 50% of ethanol. Altogether, our present work confirms the deleterious effect of ethanol on the structure of B. subtilis spores, as well as demonstrates the ability of FLIM - Bodipy-C12 to measure changes in the microviscosity of the spores upon perturbation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0006-3002 |
| Relation: | Biochimica et Biophysica Acta; http://hdl.handle.net/10044/1/34362; https://dx.doi.org/10.1016/j.bbamem.2016.06.003; EP/I003983/1 |
| DOI: | 10.1016/j.bbamem.2016.06.003 |
| الاتاحة: | http://hdl.handle.net/10044/1/34362 https://doi.org/10.1016/j.bbamem.2016.06.003 |
| Rights: | © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.6B40D302 |
| قاعدة البيانات: | BASE |
| تدمد: | 00063002 |
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| DOI: | 10.1016/j.bbamem.2016.06.003 |