التفاصيل البيبلوغرافية
| العنوان: |
Use of Light Spectra for Efficient Production of PLBs in Temperate Terrestrial Orchids |
| المؤلفون: |
Hossein Naderi Boldaji, Shirin Dianati Daylami, Kourosh Vahdati |
| المصدر: |
Horticulturae ; Volume 9 ; Issue 9 ; Pages: 1007 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
Epipactis veratifolia, Dactylorhiza umberosa, direct somatic embryogenesis, plant growth regulator, protocorm-like body, wounding |
| جغرافية الموضوع: |
agris |
| الوصف: |
Wild orchids, especially the terrestrial temperate ones are endangered species due to challenges in their natural habitats. Therefore, there is an urgent need to introduce efficient propagation methods to overcome the natural reproduction problems of these orchids. In this study, the effects of different light spectrums, explant types, wounding, and combinations of different plant growth regulators (PGRs) on direct somatic embryogenesis (DSE) of two species of these endangered orchids listed in the conservation category, were studied. The highest percentages of DSE formation and embryo germination were observed in Dactylorhiza umberosa protocorm explants exposed to white light (400–730 nm) and in Epipactis veratifolia protocorm explants exposed to a combination of red and far-red spectra (R: FR = 70:30). This occurred while red (610–700) alone and in combination with far-red (710–730 nm) spectrum induced embryogenesis more than the blue spectrum and dark condition in E. veratifolia. Thidiazuron (TDZ, 3 mg L−1), produced the highest percentage of protocorm-like bodies (PLBs) on protocorm explants in both orchids. Kinetin (Kin, 2 mg L−1) and Benzyladenine (BA 3 mg L−1) had the most effect on the survival and growth of PLBs, respectively, in D. umberosa and E. veratifolia. Species did not show similar embryogenesis responses under light spectrums. In a medium containing 3 mg L−1 TDZ, white light and R-FR spectra produced the most PLBs on wounded protocorm explants of D. umberosa and E. veratifolia respectively. The developmental stage of apical meristem of PLBs in both species was more advanced under R-B spectra, compared to others. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Propagation and Seeds; https://dx.doi.org/10.3390/horticulturae9091007 |
| DOI: |
10.3390/horticulturae9091007 |
| الاتاحة: |
https://doi.org/10.3390/horticulturae9091007 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.6B352A38 |
| قاعدة البيانات: |
BASE |