التفاصيل البيبلوغرافية
| العنوان: |
Characterization of 18-month-old Kdm6a conditional KO x Vav1 -Cre mice. |
| المؤلفون: |
Ling Tian (107492), Monique Chavez (11707313), Gue Su Chang (11707316), Nichole M. Helton (11707319), Casey D. S. Katerndahl (11707322), Christopher A. Miller (10677605), Lukas D. Wartman (11707325) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Cell Biology, Genetics, Molecular Biology, Developmental Biology, Cancer, Hematology, Biological Sciences not elsewhere classified, zheng et al, inactivation may contribute, human aml samples, develop overt leukemia, allele inactivation initiated, 2 – 15, >< sup ><, seq studies showed, histone h3k27me3 demethylase, dependent myelodysplastic syndrome, >- ko mice, myelodysplastic syndrome, dependent expansion, >, >- wt, >- knockout, >- cre, yet clear, x chromosome, vav1 <, uty <, utx < |
| الوصف: |
(A) The normal survival curve demonstrated that the inactivation of Kdm6a could not induce mice to develop overt leukemia or die of lethal abnormalities. (B) Spleen weight for all genotypes of Kdm6a conditional KO mice and appropriate littermate controls. Several KO-F mice had marked splenomegaly (> 0.5 g). (C) CBCs showed that KO-F mice had anemia and thrombocytopenia, but no difference was seen in Het-F or KO-M mice. (D) Flow cytometry analysis of mature cell lineage in the spleen from KO-F and WT-F mice. Representative flow cytometry plots showed the deletion of Kdm6a caused a striking myeloid skewing with an increased number of Gr1 + CD11b + myeloid cells and an increase in Ter119 + CD71 + erythroid precursors with age. (E) Experimental scheme of the KO-F spleen cells transplantation. Spleen cells were harvested from 3 18 months old KO-F donor mice and transplanted into non-irradiated recipient mice. PB was examined for donor cell chimerism on a monthly basis after transplantation. (F) PB donor cell chimerism indicated the donor-derived spleen cells could not engraft in recipient mice. After long-term observation, mice from each donor cohort were sacrificed and BM cells were harvested for flow cytometry analysis. Representative flow cytometric plots and quantification of donor cell chimerism in whole BM and the HSC compartment (LSK). KO-F donor HSC cells engraft and exhibited a great contribution to the SLAM compartment (14.6% in LSK cells and 50% in SLAM cells). Error bars represented mean ± s.d. *p<0.05, **p<0.01, ***p<0.001 by unpaired two-tailed Student’s t -test. (PDF) |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Characterization_of_18-month-old_i_Kdm6a_i_conditional_KO_x_i_Vav1_i_-Cre_mice_/17015408 |
| DOI: |
10.1371/journal.pone.0255706.s006 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0255706.s006 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.6ADB45A4 |
| قاعدة البيانات: |
BASE |