Academic Journal
Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting.
| العنوان: | Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting. |
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| المؤلفون: | Mainil, Jacques, Bardiau, Marjorie, Ooka, T., Ogura, Y., Murase, K., Etoh, Y., Ichihara, S., Horikawa, K., Buvens, G., Pierard, D., Itoh, T., Hayashi, T. |
| المصدر: | Journal of Applied Microbiology, 111 (3), 773-86 (2011) |
| بيانات النشر: | Blackwell Publishing |
| سنة النشر: | 2011 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Life sciences, Microbiology, Veterinary medicine & animal health, Sciences du vivant, Microbiologie, Médecine vétérinaire & santé animale |
| الوصف: | peer reviewed ; AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS AND RESULTS: Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS621 fingerprints and PFGE profiles. CONCLUSIONS: The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. SIGNIFICANCE AND IMPACT OF THE STUDY: The IS621 printing method represents a rapid (24 h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1364-5072 1365-2672 |
| Relation: | urn:issn:1364-5072; urn:issn:1365-2672; https://orbi.uliege.be/handle/2268/116010; info:hdl:2268/116010; info:pmid:21707882 |
| DOI: | 10.1111/j.1365-2672.2011.05089.x |
| الاتاحة: | https://orbi.uliege.be/handle/2268/116010 https://doi.org/10.1111/j.1365-2672.2011.05089.x |
| Rights: | restricted access ; http://purl.org/coar/access_right/c_16ec ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.6AC1420F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 13645072 13652672 |
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| DOI: | 10.1111/j.1365-2672.2011.05089.x |